Modomics - A Database of RNA Modifications

ID Card:

Full name: Ribosomal RNA large subunit methyltransferase F
Synonym: YbiN, JW5107
GI: 3915947
Orf: b0807
COG: COG3129
UniProt: P75782
Alpha Fold Predicted Structure: AF-P75782-F1
Enzyme type: methyltransferase
Position of modification - modification: l:1618(1618) - m6A



Protein sequence:

MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR

Comments:

This enzyme catalyzes the site-specific formation of m6A at position 1618 in the loop of the mini hairpin 59a in domain III of the large subunit of rRNA, located nearby the peptide tunnel of the ribosome. Recombinant RlmF protein is able to methylate partially deproteinized 50S ribosomal subunit, but neither the completely assembled 50S subunits nor completely deproteinized 23S rRNA. AdoMet is the methyl group donor. The C-terminal domain of RlmF is related to the ones of RsmC (m2G1209)/RsmD(m2G966).





Alpha Fold Predicted Structure:






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Protein sequence:

M S A Q K P G L H P R N R H H S R Y D L A T L C Q V N P E L R Q F L T L T P A G E Q S V D F A N P L A V K A L N K A L L A H F Y A V A N W D I P D G F L C P P V P G R A D Y I H H L A D L L A E A S G T I P A N A S I L D I G V G A N C I Y P L I G V H E Y G W R F T G S E T S S Q A L S S A Q A I I S S N P G L N R A I R L R R Q K E S G A I F N G I I H K N E Q Y D A T L C N P P F H D S A A A A R A G S E R K R R N L G L N K D D A L N F G G Q Q Q E L W C E G G E V T F I K K M I E E S K G F A K Q V M W F T S L V S R G E N L P P L Y R A L T D V G A V K V V K K E M A Q G Q K Q S R F I A W T F M N D E Q R R R F V N R Q R
50100150200250300SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P75782-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P75782-F1.cif  
DSSP Secondary Structures   P75782.dssp  





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