Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA pseudouridine synthase 1
GI: 3122655
Orf: YPL212C
COG: COG0101
UniProt: Q12211
Structures: | 7R9F | 7R9G |
Alpha Fold Predicted Structure: AF-Q12211-F1
Enzyme type: pseudouridine synthase
Position of modification - modification: t:34 - Y
t:36 - Y
n:44 - Y
t:35 - Y
t:27 - Y
t:28 - Y


PDB Structures:


7R9F

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   7R9F.pdb   7R9G.pdb  
Pdbx/mmCIF Files   7R9F.cif   7R9G.cif  


Protein sequence:

MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELPGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDENVFNAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFEFLTAKGIPGLTDAPESNKKIKQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN

Comments:

Multisite, multisubstrate specificity. Forms pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Modifies also U2 snRNA. Zinc enzyme. Pus1 in other organisms may exhibit different specificity.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:Y RNA snRNA 44 Nucleus
U:Y RNA tRNA 27 UAU UAU tRNAIleUAU ~ Nucleus
U:Y RNA tRNA 27 UAC UAC tRNAValUAC ~ Nucleus
U:Y RNA tRNA 27 CAC CAC tRNAValCAC ~ Nucleus
U:Y RNA tRNA 28 CAC CAC tRNAValCAC D-stem loop Nucleus
U:Y RNA tRNA 27 CCA CCA tRNATrpCCA ~ Nucleus
U:Y RNA tRNA 28 CCA CCA tRNATrpCCA D-stem loop Nucleus
U:Y RNA tRNA 67 SUU SUU tRNALysSUU acceptor-stem Nucleus
U:Y RNA tRNA 27 ACU ACU tRNAArgACU ~ Nucleus
U:Y RNA tRNA 27 ICG ICG tRNAArgICG ~ Nucleus
U:Y RNA tRNA 27 CUC CUC tRNAGluCUC ~ Nucleus
U:Y RNA tRNA 27 CUU CUU tRNALysCUU ~ Nucleus
U:Y RNA tRNA 27 SUU SUU tRNALysSUU ~ Nucleus
U:Y RNA tRNA 28 SUU tRNALysSUU anticodon-stem loop Nucleus
U:Y RNA tRNA 27 CAU CAU tRNAMetCAU D-stem loop Nucleus
U:Y RNA tRNA 35 GUA GYA tRNATyrGUA anticodon Nucleus
U:Y RNA tRNA 65 CCA CCA tRNATrpCCA acceptor-stem Nucleus
U:Y RNA tRNA 67 UAU UAU tRNAIleUAU acceptor-stem Nucleus
U:Y RNA tRNA 26 CCA CCA tRNATrpCCA D-stem loop Nucleus
U:Y RNA pre-tRNA 27 UAU UAU tRNAIleUAU ~ Nucleus
U:Y RNA pre-tRNA 34 UAU YAU tRNAIleUAU wobble - position Nucleus
U:Y RNA pre-tRNA 36 UAU UAY tRNAIleUAU anticodon Nucleus
U:Y RNA tRNA 1 ICG ICG tRNAArgICG D-stem loop Nucleus
U:Y RNA tRNA 26 BCA BCA tRNATrpBCA D-loop stem Nucleus

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S E E N L R P A Y D D Q V N E D V Y K R G A Q S K L T K A R K A D F D D E K D K K K D N D K H I D K R P K S G P R L D E N G N P L P K E P R L P K R K V A V M V G Y C G T G Y H G M Q Y N P P N P T I E S A L F K A F V E A G A I S K D N S N D L K K N G F M R A A R T D K G V H A G G N L I S L K M I I E D P D I K Q K I N E K L P E G I R V W D I E R V N K A F D C R K M C S S R W Y E Y L L P T Y S L I G P K P G S I L Y R D I E E S K T E L P G V L D E D L E S K E F W E E F K K D A N E K F S T E E I E A I L A Y V P P A R D E F D I N E E L Y Q K V K K Y K Q L E N A H R R R Y R I S A A K L A K F R A S T S Q Y L G A H N F H N F T L G K D F K E P S A I R F M K D I K V S D P F V I G D A Q T E W I S I K I H G Q S F M L H Q I R K M V S M A T L I T R C G C P V E R I S Q A Y G Q Q K I N I P K A P A L G L L L E A P V F E G Y N K R L E Q F G Y K A I D F S K Y Q D E V D K F K M K H I Y D K I Y K E E V D E N V F N A F F S Y I D S F N K V T G A Q G E E T A D K S G P A V Q K S I F E F L T A K G I P G L T D A P E S N K K I K Q R K R M E E E E A A S K K A E I S S T T Q S N E P E V Q P E A A A N

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q12211-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q12211-F1.cif  
DSSP Secondary Structures   Q12211.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. Motorin Y, Keith G, Simon C, Foiret D, Simos G, Hurt E, Grosjean H RNA [details] 9671058 -
Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Massenet S, Motorin Y, Lafontaine DL, Hurt EC, Grosjean H, Branlant C Mol Cell Biol [details] 10022901 -
A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs. Behm-Ansmant I, Massenet S, Immel F, Patton JR, Motorin Y, Branlant C RNA [details] 16804160 -
RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA(Phe). Arluison V, Batelier G, Riès-Kautt M, Grosjean H Biochimie [details] 10492022 -
Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. Arluison V, Buckle M, Grosjean H J Mol Biol [details] 10356324 -
Transfer RNA-pseudouridine synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition. Arluison V, Hountondji C, Robert B, Grosjean H Biochemistry [details] 9585540 -