Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA pseudouridine synthase 1
GI: 3122655
Orf: YPL212C
COG: COG0101
UniProt: Q12211
Structures: | 7R9F | 7R9G |
Alpha Fold Predicted Structure: AF-Q12211-F1
Enzyme type: pseudouridine synthase
Position of modification - modification: t:34 - Y
t:36 - Y
n:44 - Y
t:35 - Y
t:27 - Y
t:28 - Y


PDB Structures:


7R9F

Structure Description:

Title: Wild-type yeast Pseudouridine Synthase, PUS1, bound to 5-Fluorouracil RNA
Classification: RNA BINDING PROTEIN/RNA
Technique: X-Ray Diffraction
Resolution: 2.89
R value free: 0.290
R value observed: 0.255
R value work: 0.251

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   7R9F.pdb   7R9G.pdb  
Pdbx/mmCIF Files   7R9F.cif   7R9G.cif  


Protein sequence:

MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELPGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDENVFNAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFEFLTAKGIPGLTDAPESNKKIKQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN

Comments:

Multisite, multisubstrate specificity. Forms pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Modifies also U2 snRNA. Zinc enzyme. Pus1 in other organisms may exhibit different specificity.





Alpha Fold Predicted Structure:




Parsing response... [502816/502816]


Clear Selection and Reset Camera

Protein sequence:

M S E E N L R P A Y D D Q V N E D V Y K R G A Q S K L T K A R K A D F D D E K D K K K D N D K H I D K R P K S G P R L D E N G N P L P K E P R L P K R K V A V M V G Y C G T G Y H G M Q Y N P P N P T I E S A L F K A F V E A G A I S K D N S N D L K K N G F M R A A R T D K G V H A G G N L I S L K M I I E D P D I K Q K I N E K L P E G I R V W D I E R V N K A F D C R K M C S S R W Y E Y L L P T Y S L I G P K P G S I L Y R D I E E S K T E L P G V L D E D L E S K E F W E E F K K D A N E K F S T E E I E A I L A Y V P P A R D E F D I N E E L Y Q K V K K Y K Q L E N A H R R R Y R I S A A K L A K F R A S T S Q Y L G A H N F H N F T L G K D F K E P S A I R F M K D I K V S D P F V I G D A Q T E W I S I K I H G Q S F M L H Q I R K M V S M A T L I T R C G C P V E R I S Q A Y G Q Q K I N I P K A P A L G L L L E A P V F E G Y N K R L E Q F G Y K A I D F S K Y Q D E V D K F K M K H I Y D K I Y K E E V D E N V F N A F F S Y I D S F N K V T G A Q G E E T A D K S G P A V Q K S I F E F L T A K G I P G L T D A P E S N K K I K Q R K R M E E E E A A S K K A E I S S T T Q S N E P E V Q P E A A A N
50100150200250300350400450500SequenceGHITBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q12211-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q12211-F1.cif  
DSSP Secondary Structures   Q12211.dssp  





Publications: