Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA pseudouridine synthase 2
GI: 347595823
Orf: YGL063W
COG: COG0101
UniProt: P53167
Alpha Fold Predicted Structure: AF-P53167-F1
Enzyme type: pseudouridine synthase
Position of modification - modification: t:27 - Y



Protein sequence:

MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAPKKNSFMAAARTDKGVHAMLNLLSLKITLREDTVAKLNAALPPEIRVWGIQPVNKKFNARSACDSRWYQYLIPEFILIGPPRSSLLHRNVGGSYREDGSQEVWDTFLEQTRGRFSGDELCRLQDTAQKLSESDPLVQDYVGLLSGTLSGYCLSPSKLDAFEAAMQEYVGTHNFHNFTTGKLWGDPSAQRHIKKVVVSQASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQLPPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVFDGYNTKLRNLLYCEIRPDDITLERMCRFRERQICTAIAHEETQRHVFCHFVRQMNRLATPLI

Comments:

Specificity unpublished




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:Y RNA tRNA 26 UCU UCU tRNAArgUCU D-stem loop Mitochondrion
U:Y RNA tRNA 26 ACG ACG tRNAArgACG D-stem loop Mitochondrion
U:Y RNA tRNA 26 GUG GUG tRNAHisGUG D-stem loop Mitochondrion
U:Y RNA tRNA 26 UAG UAG tRNASerUAG D-stem loop Mitochondrion
U:Y RNA tRNA 26 UAG UAG tRNAThrUAG D-stem loop Mitochondrion
U:Y RNA tRNA 26 GUA GUA tRNATyrGUA D-stem loop Mitochondrion
U:Y RNA tRNA 27 UUU UUU tRNALysUUU ~ Mitochondrion

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M L L G Y C G S G Y Y G M Q Y N P P H K T I E G E I L T K L F D V G A I S E E N S L A P K K N S F M A A A R T D K G V H A M L N L L S L K I T L R E D T V A K L N A A L P P E I R V W G I Q P V N K K F N A R S A C D S R W Y Q Y L I P E F I L I G P P R S S L L H R N V G G S Y R E D G S Q E V W D T F L E Q T R G R F S G D E L C R L Q D T A Q K L S E S D P L V Q D Y V G L L S G T L S G Y C L S P S K L D A F E A A M Q E Y V G T H N F H N F T T G K L W G D P S A Q R H I K K V V V S Q A S P G W I C V R I H G Q S F M L H Q I R R M V A L A V L A A R C Q L P P N I V R N Y F N A G P R K Y I P R A P A Q G L L L E G P V F D G Y N T K L R N L L Y C E I R P D D I T L E R M C R F R E R Q I C T A I A H E E T Q R H V F C H F V R Q M N R L A T P L I

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P53167-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P53167-F1.cif  
DSSP Secondary Structures   P53167.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase. Behm-Ansmant I, Branlant C, Motorin Y RNA [details] 17684231 -