Modomics - A Database of RNA Modifications

Full name:	tRNA pseudouridine synthase 3
Synonym:	Deg1p, Hrm3
GI:	399356
Orf:	YFL001W
COG:	COG0101
UniProt:	P31115
Structures:	\| \|
Complex:
Enzyme type:	pseudouridine synthase
Position of modification - modification:	t:39 - Y t:38 - Y

Comments:

Region specificity.Monomeric enzyme.

Protein sequence:

MSNFIRRLVGKMKAISTGTNAIVSKKDSIYANWSKEQLIRRITELENANKPHSEKFQHIEDNKKRKISQEEVTRSKAKKAPKKFDFSKHNTRFIALRFAY
LGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLTDEEQRDPTNDSREIPYVHVLNQLLPDDIRI
SAVCLRPPPNFDARFSCVHRHYKYIFNGKNLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPLSETFYCFDLVGSAFLWHQVRCMMA
ILFLVGQSLEVPEIVLRLTDIEKTPQRPVYEMANDIPLLLYDCKFPEMDWQEPTVDDYKAIKFTTATEALTLHYELKAAVCNIFKDVLPTANTNNFSKTI
INLGDGRGKVVGTYVKLEDRSVMEPVEVVNAKYSKKKNNKNK

Enzymatic activities:

Reaction	Substrate	Type	Position
U:Y	tRNA (t)	Ala/IGC/prokaryotic cytosol	38
U:Y	tRNA (t)	Gly/GCC/prokaryotic cytosol	38
U:Y	tRNA (t)	Arg/ACU/prokaryotic cytosol	39
U:Y	tRNA (t)	Asn/GUU/prokaryotic cytosol	39
U:Y	tRNA (t)	Cys/GCA/prokaryotic cytosol	39
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	39
U:Y	tRNA (t)		39
U:Y	tRNA (t)	Leu/CAA/prokaryotic cytosol	39
U:Y	tRNA (t)		39
U:Y	tRNA (t)	Lys/CUU/prokaryotic cytosol	39
U:Y	tRNA (t)	Met/CAU/prokaryotic cytosol	39
U:Y	tRNA (t)	Phe/GAA/prokaryotic cytosol	39
U:Y	tRNA (t)	Ser/CGA/prokaryotic cytosol	39
U:Y	tRNA (t)	Ser/UGA/prokaryotic cytosol	39
U:Y	tRNA (t)	Ser/IGA/prokaryotic cytosol	39
U:Y	tRNA (t)	Thr/IGU/prokaryotic cytosol	39
U:Y	tRNA (t)	Trp/CCA/prokaryotic cytosol	39
U:Y	tRNA (t)	Tyr/GUA/prokaryotic cytosol	39
U:Y	tRNA (t)	His/GUG/mitochondrion	38
U:Y	tRNA (t)	Thr/UAG/mitochondrion	38
U:Y	tRNA (t)	Arg/UCU/mitochondrion	39
U:Y	tRNA (t)	Arg/ACG/mitochondrion	39
U:Y	tRNA (t)	Leu/UAA/mitochondrion	39
U:Y	tRNA (t)	Phe/GAA/mitochondrion	39
U:Y	tRNA (t)	Ser/UGA/mitochondrion	39
U:Y	tRNA (t)	Trp/UCA/mitochondrion	39

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop.	Lecointe F, Simos G, Sauer A, Hurt EC, Motorin Y, Grosjean H	J Biol Chem	[details]	9430663	-