Modomics - A Database of RNA Modifications

Full name:	tRNA pseudouridine synthase 4
Synonym:
GI:	1353109
Orf:	YNL292W
COG:	COG0130
UniProt:	P48567
Structures:	\| \|
Complex:
Enzyme type:	pseudouridine synthase
Position of modification - modification:	t:55 - Y

Comments:

Acts on mitochondrial and cytoplasmic tRNAs. Monomeric enzyme.

Protein sequence:

MNGIFAIEKPSGITSNQFMLKLQHALTKSQVFSKEIQRATAERKQQYEKQTGKKASKRKLRKVSKVKMGHGGTLDPLASGVLVIGIGAGTKKLANYLSGT
VKVYESEALFGVSTTSGDVEGEILSQNSVKHLNFDDLKTVEEKFVGQLKQTPPIYAALKMDGKPLHEYAREGKPLPRAIEPRQVTIYDLKVFSDSLKRDH
DYPLLRPTTEEAVDTVKNLNANMLNDVLYFSKEYTEKHGLDSEVAKVEEPFPLSEQEEQEIQKEGDSYRAPKLHFKANVSSGTYIRSLVSDIGKSMRSSC
YMVKLIRLQQQDWSLEKNNVFQLTDFTERDEKVWSKVLEKVLDEGATVDVIEELKKAEKEIPADVKECIVSSDQPGDEATAETIETANAEEHSNTLKRKI
EQV

Enzymatic activities:

Reaction	Substrate	Type	Position
U:Y	tRNA (t)	Ala/IGC/prokaryotic cytosol	55
U:Y	tRNA (t)	Arg/ACU/prokaryotic cytosol	55
U:Y	tRNA (t)	Arg/ICG/prokaryotic cytosol	55
U:Y	tRNA (t)	Asn/GUU/prokaryotic cytosol	55
U:Y	tRNA (t)	Asp/GUC/prokaryotic cytosol	55
U:Y	tRNA (t)	Cys/GCA/prokaryotic cytosol	55
U:Y	tRNA (t)	Glu/CUC/prokaryotic cytosol	55
U:Y	tRNA (t)	Gly/GCC/prokaryotic cytosol	55
U:Y	tRNA (t)	Gly/{CC/prokaryotic cytosol	55
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	55
U:Y	tRNA (t)	Ile/IAU/prokaryotic cytosol	55
U:Y	tRNA (t)	Ile/UAU/prokaryotic cytosol	55
U:Y	tRNA (t)	Leu/UAG/prokaryotic cytosol	55
U:Y	tRNA (t)	Leu/CAA/prokaryotic cytosol	55
U:Y	tRNA (t)	Leu/UAA/prokaryotic cytosol	55
U:Y	tRNA (t)	Lys/CUU/prokaryotic cytosol	55
U:Y	tRNA (t)	Lys/SUU/prokaryotic cytosol	55
U:Y	tRNA (t)	Met/CAU/prokaryotic cytosol	55
U:Y	tRNA (t)	Phe/GAA/prokaryotic cytosol	55
U:Y	tRNA (t)	Pro/UGG/prokaryotic cytosol	55
U:Y	tRNA (t)	Ser/CGA/prokaryotic cytosol	55
U:Y	tRNA (t)	Ser/UGA/prokaryotic cytosol	55
U:Y	tRNA (t)	Ser/IGA/prokaryotic cytosol	55
U:Y	tRNA (t)	Thr/IGU/prokaryotic cytosol	55
U:Y	tRNA (t)	Trp/CCA/prokaryotic cytosol	55
U:Y	tRNA (t)	Tyr/GUA/prokaryotic cytosol	55
U:Y	tRNA (t)	Val/UAC/prokaryotic cytosol	55
U:Y	tRNA (t)	Val/CAC/prokaryotic cytosol	55
U:Y	tRNA (t)	Val/IAC/prokaryotic cytosol	55
U:Y	tRNA (t)	Arg/UCU/mitochondrion	55
U:Y	tRNA (t)	Arg/ACG/mitochondrion	55
U:Y	tRNA (t)	Gly/UCC/mitochondrion	55
U:Y	tRNA (t)	His/GUG/mitochondrion	55
U:Y	tRNA (t)	Ile/GAU/mitochondrion	55
U:Y	tRNA (t)	Leu/UAA/mitochondrion	55
U:Y	tRNA (t)	Lys/UUU/mitochondrion	55
U:Y	tRNA (t)	Met/CAU/mitochondrion	55
U:Y	tRNA (t)	Phe/GAA/mitochondrion	55
U:Y	tRNA (t)	Pro/UGG/mitochondrion	55
U:Y	tRNA (t)	Ser/UGA/mitochondrion	55
U:Y	tRNA (t)	Ser/GCU/mitochondrion	55
U:Y	tRNA (t)	Thr/UAG/mitochondrion	55
U:Y	tRNA (t)	Trp/UCA/mitochondrion	55
U:Y	tRNA (t)	Tyr/GUA/mitochondrion	55
U:Y	tRNA (t)		55

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs.	Becker HF, Motorin Y, Planta RJ, Grosjean H	Nucleic Acids Res	[details]	9358157	-