Modomics - A Database of RNA Modifications

ID Card:

Full name:	Multisubstrate pseudouridine synthase 7
GI:	6324817
Orf:	YOR243C
COG:	COG0585
UniProt:	Q08647
Structures:	\| \|
Enzyme type:	pseudouridine synthase
Position of modification - modification:	s:many - Y n:35 - Y t:13 - Y t:35 - Y n:56 - Y

Protein sequence:

MSDSSEATVKRPLDAHVGPSENAAKKLKIEQRTQADGIHEADVGITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKEEVNAEKESEAARRQEFNVDPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKIHQLLREAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKDFIHFTLHKENKDTMEAVNVITKLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLNALNRTLKGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTGNSEVSLEEIVSNGCKSLSENGFINYFGMQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAALALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMYVHAYQSYVWNSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFIRAKAVTQEDIDSVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRKVRDFSLAGSYRTVIQKPKSLEYRIIHYDDPSQQLVNTDLDILNNTRAKESGQKYMKAKLDRYMPDKGGEKTAVVLKFQLGTSAYATMALRELMKLETSRRGDMCDVKENI

Comments:

Cytoplasmic. Formation of pseudouridine 35 in tRNA in yeast as well as in Archaea is intron dependent. In yeast it also catalyzes formation of pseudouridine in U2 snRNA: position 35 in modified constitutively while position 56 only under stress conditions .

Search:

Reaction	Substrate	SubstrateType	Position	References
U:Y	tRNA (t)	Asp/GUC/prokaryotic cytosol	13
U:Y	tRNA (t)	Glu/CUC/prokaryotic cytosol	13
U:Y	tRNA (t)	Gly/GCC/prokaryotic cytosol	13
U:Y	tRNA (t)	Gly/{CC/prokaryotic cytosol	13
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	13
U:Y	tRNA (t)	Pro/UGG/prokaryotic cytosol	13
U:Y	tRNA (t)	Val/CAC/prokaryotic cytosol	13
U:Y	tRNA (t)	Val/IAC/prokaryotic cytosol	13
U:Y	pre-tRNA (pre-t)	Tyr/GUA/prokaryotic cytosol	35
U:Y	snRNA (sn)	U2//	35
U:Y	snRNA (sn)	U2//	56	21131909


U:Y
U:Y
U:Y
U:Y
U:Y
U:Y
U:Y
U:Y
U:Y
U:Y
U:Y

Showing 1 to 11 of 11 entries

Publications:

Search:

Title	Authors	Journal	Details	PubMed Id	DOI
Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.	Decatur WA, Schnare MN	Mol Cell Biol	[details]	18332121	-
Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae.	Yang C, McPheeters DS, Yu YT	J Biol Chem	[details]	15611063	-
RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p.	Urban A, Behm-Ansmant I, Branlant C, Motorin Y	J Biol Chem	[details]	19114708	-
The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs.	Behm-Ansmant I, Urban A, Ma X, Yu YT, Motorin Y, Branlant C	RNA	[details]	14561887	-
U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.	Wu G, Xiao M, Yang C, Yu YT...	EMBO J	[details]	21131909	-
X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.	Lin Y, Kielkopf CL	Biochemistry	[details]	18435545	-


Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.
Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae.
RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p.
The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs.
U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.
X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.

Showing 1 to 6 of 6 entries