MSDSSEATVKRPLDAHVGPSENAAKKLKIEQRTQADGIHEADVGITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKEEVNAEKESEAARRQEFNVDPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKIHQLLREAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKDFIHFTLHKENKDTMEAVNVITKLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLNALNRTLKGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTGNSEVSLEEIVSNGCKSLSENGFINYFGMQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAALALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMYVHAYQSYVWNSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFIRAKAVTQEDIDSVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRKVRDFSLAGSYRTVIQKPKSLEYRIIHYDDPSQQLVNTDLDILNNTRAKESGQKYMKAKLDRYMPDKGGEKTAVVLKFQLGTSAYATMALRELMKLETSRRGDMCDVKENI
Cytoplasmic. Formation of pseudouridine 35 in tRNA in yeast as well as in Archaea is intron dependent. In yeast it also catalyzes formation of pseudouridine in U2 snRNA: position 35 in modified constitutively while position 56 only under stress conditions .
| Reaction | Substrate | SubstrateType | Position | (Anti)Codon | Modified (Anti)Codon | Amino Acid Change | Transcript Name | Transcript Region | Cellular Localization | References |
|---|---|---|---|---|---|---|---|---|---|---|
| U:Y | tRNA (t) | Asp/GUC/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Glu/CUC/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Gly/GCC/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Gly/{CC/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | His/GUG/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Pro/UGG/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Val/CAC/prokaryotic cytosol | 13 | |||||||
| U:Y | tRNA (t) | Val/IAC/prokaryotic cytosol | 13 | |||||||
| U:Y | pre-tRNA (pre-t) | Tyr/GUA/prokaryotic cytosol | 35 | |||||||
| U:Y | snRNA (sn) | U2// | 35 | |||||||
| U:Y | snRNA (sn) | U2// | 56 | 21131909 |
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. | Behm-Ansmant I, Urban A, Ma X, Yu YT, Motorin Y, Branlant C | RNA | [details] | 14561887 | - |
| Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae. | Yang C, McPheeters DS, Yu YT | J Biol Chem | [details] | 15611063 | - |
| X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines. | Lin Y, Kielkopf CL | Biochemistry | [details] | 18435545 | - |
| Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs. | Decatur WA, Schnare MN | Mol Cell Biol | [details] | 18332121 | - |
| RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p. | Urban A, Behm-Ansmant I, Branlant C, Motorin Y | J Biol Chem | [details] | 19114708 | - |
| U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP. | Wu G, Xiao M, Yang C, Yu YT... | EMBO J | [details] | 21131909 | - |