Modomics - A Database of RNA Modifications

Full name:	Bifunctional protein RIB2
Synonym:	Rib2
GI:	2501674
Orf:	YOL066c
COG:	COG0564
UniProt:	Q12362
Structures:	\| \|
Complex:
Enzyme type:	pseudouridine synthase/deaminase
Position of modification - modification:	t:32 - Y

Comments:

Cytoplasmic enzyme (mitochondrial homologue is Pus9). N-terminal part is Rib2p fused protein (not in all organisms).

Protein sequence:

MEDSNNEASDDFNNLLNKEIESAKEVKLRKFANRNNNRNENSSKVKDASGFRLRVIQTDGHKTKKTDPDYEVTIDGPLRKIEPYFFTYKTFCKERWRDRK
LVDVFVSEFRDREPSYYSKTIAEGKVYLNDEPANLDTIIRDGDLITHKVHRHEPPVTSKPIDIVFEDEDILVIDKPSSIPVHPTGRYRFNTITKMLERQL
GYSVHPCNRLDKPTSGLMFLAKTPLGADRMGDQMKAREVTKEYVARVKGEFPIGIVEVDKPVRSVNPKVALNAVCEMSDENAKHAKTVFQRVSYDGQTSI
VKCKPLTGRTHQIRVHLQYLGFPIANDPIYSNPDIWGPDLGRGGLQNYDDIVLKLDAIGKTNPAESWIHPHSEGEYLLGRQCEECEAEMYTDPGTNDLDL
WLHAFRYESLERNSDTQKPLWSYRTKYPEWALEPHRRYMEMAVKEAGKCGPTKTAFSVGAVLVHGTQVLATGYSRELPGNTHAEQCALIKYSQLHPNCPT
IVPMGTVLYTTMEPCSFRLSGNEPCCDRILATQGAIGTVFVGVMEPDTFVKNNTSLNKLESHGVNYIQIPGYEEECTIIAFKGHDNSDDKA

Enzymatic activities:

Reaction	Substrate	Type	Position
U:Y	tRNA (t)	Asp/GUC/prokaryotic cytosol	32
U:Y	tRNA (t)	Cys/GCA/prokaryotic cytosol	32
U:Y	tRNA (t)	Gly/GCC/prokaryotic cytosol	32
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	32
U:Y	tRNA (t)	Leu/UAG/prokaryotic cytosol	32
U:Y	tRNA (t)	Leu/CAA/prokaryotic cytosol	32
U:Y	tRNA (t)	Pro/UGG/prokaryotic cytosol	32
U:Y	tRNA (t)	Ser/IGA/prokaryotic cytosol	32
U:Y	tRNA (t)	Val/UAC/prokaryotic cytosol	32
U:Y	tRNA (t)	Val/CAC/prokaryotic cytosol	32
U:Y	tRNA (t)	Val/IAC/prokaryotic cytosol	32

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.	Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C	J Biol Chem	[details]	15466869	-