Modomics - A Database of RNA Modifications

Full name:	tRNA pseudouridine synthase 9, mitochondrial
Synonym:
GI:	6320168
Orf:	YDL036c
COG:	COG0564
UniProt:	Q12069
Structures:	\| \|
Complex:
Enzyme type:	pseudouridine synthase
Position of modification - modification:	t:32 - Y

Comments:

Mitochondrial enzyme. In the absence of Pus8 works also in cytoplasm.

Protein sequence:

MQRNNRLRNLFTVPVIMARQLKRNALSAGLAFAGNATSNEFDEHLQNEVEREREIQKKKKIKRTQSKKSPDLINKSTFQSRTIGSKKEKHRQLDPEYEIV
IDGPLRKIKPYHFTYRTFCKERWRDKKLVDVFISEFRDRESEYYKRTIENGDVHINDETADLSTVIRNGDLITHQVHRHEPPVTSRPIKVIFEDDNIMVI
DKPSGIPVHPTGRYRFNTITKMLQNNLGFVVNPCNRLDRLTSGLMFLAKTPKGADNIGDQLKAREVTKEYVAKVVGEFPETEVIVEKPLKLIEPRLALNA
VCQMDEKGAKHAKTVFNRISYDGKTSIVKCKPLTGRSHQIRVHLQYLGHPIANDPIYSNDEVWGNNLGKGGQADFDIVITKLDEIGKRKPAKSWFHSNGG
YGEVLRQEKCSICESDLYTDPGPNDLDLWLHAYLYESTETEEGTEKKKWCYKTEYPEWALRR

Enzymatic activities:

Reaction	Substrate	Type	Position
U:Y	tRNA (t)	His/GUG/mitochondrion	32
U:Y	tRNA (t)	Phe/GAA/mitochondrion	32
U:Y	tRNA (t)	Pro/UGG/mitochondrion	32
U:Y	tRNA (t)	Ser/UGA/mitochondrion	32
U:Y	tRNA (t)	Ser/GCU/mitochondrion	32
U:Y	tRNA (t)	Trp/UCA/mitochondrion	32

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.	Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C	J Biol Chem	[details]	15466869	-