Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA guanosine-2′-O-methyltransferase TRM11
GI: 74655048
Orf: YOL124C
COG: COG1041
UniProt: Q12463
Alpha Fold Predicted Structure: AF-Q12463-F1
Enzyme type: methyltransferase
Position of modification - modification: t:10 - m2G



Protein sequence:

MKKYLLYMVQVHLNFRRAELESLADLYNLSIDFSQYDANSPFFIVELENDQQAKDWIKRSILTRGIYEYWGQGTTLDELHKDIQRQSNFEQDLQLKFKHSTFKFEFECYKGNSKAKRVEQIETFRYLGFEGKIDMKHPQEVFTVIEEYTPISENVGGKTPTRIYFGRQVQMSNRSAMEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPGTIMYDPFAGTGSFLVAGGHFGSLVIGSDIDGRMIRGKGAQVNISANFKKYGESSQFLDVLTMDFTNNALRNNLVIDTILCDPPYGIRESIKVLGAKDPERFLGKEDMEIDGEKAYLRRDYIPTKKPYALDSLLDDLLQYSSERLPIGGRLAFWMPTANDANIETIVPMHENLELKYNCVQEFNKWSRRLLVYINRGSTFNGSSNHGIKRSKDNFRERYFNNFN

Comments:

AdoMet is the methyl group donor. Heterodimer composed of two unrelated subunits: Trm11p (catalytic subunit) and Trm112p (essential cofactor). Contains a THUMP domain.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m2G RNA tRNA 10 ACU ACU tRNAArgACU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 ICG ICG tRNAArgICG D-stem loop Cytoplasm
G:m2G RNA tRNA 10 GUU GUU tRNAAsnGUU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 IAU IAU tRNAIleIAU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 UAU UAU tRNAIleUAU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 UAG UAG tRNALeuUAG D-stem loop Cytoplasm
G:m2G RNA tRNA 10 CAA CAA tRNALeuCAA D-stem loop Cytoplasm
G:m2G RNA tRNA 10 UAA UAA tRNALeuUAA D-stem loop Cytoplasm
G:m2G RNA tRNA 10 CUU CUU tRNALysCUU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 CAU CAU tRNAMetCAU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 GAA GAA tRNAPheGAA D-stem loop Cytoplasm
G:m2G RNA tRNA 10 IGU IGU tRNAThrIGU D-stem loop Cytoplasm
G:m2G RNA tRNA 10 CCA CCA tRNATrpCCA D-stem loop Cytoplasm 15899842   
G:m2G RNA tRNA 10 GUA GUA tRNATyrGUA D-stem loop Cytoplasm 15899842   
G:m2G RNA tRNA 10 CAC CAC tRNAValCAC D-stem loop Cytoplasm 15899842   
G:m2G RNA tRNA 10 UAC UAC tRNAValUAC D-stem loop Cytoplasm 15899842   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K K Y L L Y M V Q V H L N F R R A E L E S L A D L Y N L S I D F S Q Y D A N S P F F I V E L E N D Q Q A K D W I K R S I L T R G I Y E Y W G Q G T T L D E L H K D I Q R Q S N F E Q D L Q L K F K H S T F K F E F E C Y K G N S K A K R V E Q I E T F R Y L G F E G K I D M K H P Q E V F T V I E E Y T P I S E N V G G K T P T R I Y F G R Q V Q M S N R S A M E K Y D L K K R P Y K G T T S F E A E L S L V S A N I A Q V K P G T I M Y D P F A G T G S F L V A G G H F G S L V I G S D I D G R M I R G K G A Q V N I S A N F K K Y G E S S Q F L D V L T M D F T N N A L R N N L V I D T I L C D P P Y G I R E S I K V L G A K D P E R F L G K E D M E I D G E K A Y L R R D Y I P T K K P Y A L D S L L D D L L Q Y S S E R L P I G G R L A F W M P T A N D A N I E T I V P M H E N L E L K Y N C V Q E F N K W S R R L L V Y I N R G S T F N G S S N H G I K R S K D N F R E R Y F N N F N

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q12463-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q12463-F1.cif  
DSSP Secondary Structures   Q12463.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA. Purushothaman SK, Bujnicki JM, Grosjean H, Lapeyre B Mol Cell Biol [details] 15899842 -