Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (guanosine(18)-2′-O)-methyltransferase
Synonym: SpoU
GI: 84029292
Orf: z5077, ECs4526, SF3691, S4078, b3651
COG: COG0566
UniProt: P0AGJ2
Alpha Fold Predicted Structure: AF-P0AGJ2-F1
Enzyme type: methyltransferase
Position of modification - modification: t:18 - Gm



Protein sequence:

MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG

Comments:

AdoMet is the methyl group donor. SPOUT superfamily.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:Gm RNA tRNA 18 UUG UUG tRNAGlnUUG D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 CUG CUG tRNAGlnCUG D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 CAU CAU tRNAIleCAU D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 CAG CAG tRNALeuCAG D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 GAG GAG tRNALeuGAG D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 UAA UAA tRNALeuUAA D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 CAU CAU tRNAMetCAU D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 UGA UGA tRNASerUGA D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 CGA CGA tRNASerCGA D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 GGA GGA tRNASerGGA D-loop Prokaryotic Cytosol
G:Gm RNA tRNA 18 GUA GUA tRNATyrGUA D-loop Prokaryotic Cytosol

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M N P T R Y A R I C E M L A R R Q P D L T V C M E Q V H K P H N V S A I I R T A D A V G V H E V H A V W P G S R M R T M A S A A A G S N S W V Q V K T H R T I G D A V A H L K G Q G M Q I L A T H L S D N A V D F R E I D Y T R P T C I L M G Q E K T G I T Q E A L A L A D Q D I I I P M I G M V Q S L N V S V A S A L I L Y E A Q R Q R Q N A G M Y L R E N S M L P E A E Q Q R L L F E G G Y P V L A K V A K R K G L P Y P H V N Q Q G E I E A D A D W W A T M Q A A G

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0AGJ2-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0AGJ2-F1.cif  
DSSP Secondary Structures   P0AGJ2.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity. Persson BC, Jager G, Gustafsson C Nucleic Acids Res [details] 9321663 -
The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and non-substrate tRNA via an induced-fit process. Ochi A, Makabe K, Yamagami R, Hirata A, Sakaguchi R, Hou YM, Watanabe K, Nureki O, Kuwajima K, Hori H... J Biol Chem [details] 23867454 -