Modomics - A Database of RNA Modifications

Published on Jan. 1, 2020 in volume (2020) Mol Cell 79: 615-628.e5.

PubMed ID: 32668200

DOI: 10.1016/j.molcel.2020.06.032


Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix1 2 -Ipi3 2 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodelling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodelled by assembly factors, thereby ensuring failsafe assembly progression.