Published on Jan. 1, 1997 in Nucleosides and Nucleotides volume Volume 16, Issue 7-9.

DOI: 10.1080/07328319708006226

Abstract:

A short multigram synthesis of 2′-O-methylpseudouridine and its phosphoramidite derivative is described which avoids the use of protecting groups on the nitrogens. A binding study of oligonucleotides containing this modification suggest an increased binding affinity to RNA when compared to oligonucleotides incorporating 2′-O-methyluridine.