Published on Jan. 1, 1997 in Nucleosides and Nucleotides volume 16:7-9, 1589-1598.
We have investigated the use of alkylamines as fast cleavage and deprotection reagents for the solid phase synthesis of oligonucleotides and found methylaminelammonium hydroxide (or methylamine) as an efficient reagent. The transamination side product formed with the commonly used dCk has been eliminated by the use of dCACp hosphoramidite. This system has successfully been used in the synthesis of oligonucleotides and oligonucleoside phosphorothioates. DMT dCAc hydrogen phosphonate and DMT ribo CA'-2'-0Me phosphoramidite also have been prepared and used in the synthesis of oligonucleotides.