Summary
| Full name | wybutosine |
| IUPAC name | methyl 4-[3-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-4,6-dimethyl-9-oxoimidazo[1,2-a]purin-7-yl]-2-(methoxycarbonylamino)butanoate |
| Short name | yW |
| MODOMICS code new | 2000003483G |
| MODOMICS code | 3483G |
| Synonyms |
2-(Methoxycarbonylamino)-4-[3-(beta-D-ribofuranosyl)-4,6-dimethyl-9-oxo-4,9-dihydro-3H-imidazo[1,2-a]purine-7-yl]butyric acid methyl ester
|
| Nature of the modified residue | Natural |
| RNAMods code | Y |
| Residue unique ID | 30 |
| Found in RNA | Yes |
| Related nucleotides | 244 |
| Enzymes |
TYW4 (Saccharomyces cerevisiae) |
| Found in phylogeny | Eukaryota |
| Found naturally in RNA types | rRNA, tRNA |
Chemical information
| Sum formula | C21H28N6O9 |
| Type of moiety | nucleoside |
| Degeneracy | not applicable |
| SMILES | COC(NC(C(OC)=O)CCc1[n]2c([n](c3c(c2=O)nc[n]3[C@H]2[C@H](O)[C@H](O)[C@@H](CO)O2)C)nc1C)=O |
| logP | -1.4763 |
| TPSA | 191.67 |
| Number of atoms | 36 |
| Number of Hydrogen Bond Acceptors 1 (HBA1) | 12 |
| Number of Hydrogen Bond Acceptors 2 (HBA2) | 15 |
| Number of Hydrogen Bond Donors (HBD) | 4 |
| PDB no exac match , link to the most similar ligand | YG |
| HMDB (Human Metabolome Database) no exact match, link to the most similar ligand | None |
| InChI | InChI=1S/C21H28N6O9/c1-9-11(6-5-10(19(32)34-3)24-21(33)35-4)27-17(31)13-16(25(2)20(27)23-9)26(8-22-13)18-15(30)14(29)12(7-28)36-18/h8,10,12,14-15,18,28-30H,5-7H2,1-4H3,(H,24,33)/t10?,12-,14-,15-,18-/m1/s1 |
| InChIKey | QAOHCFGKCWTBGC-BTBIENRZSA-N |
| Search the molecule in external databases | ChEMBL ChemAgora ChEBI PubChem Compound Database Ligand Expo ChemSpider WIPO |
| PubChem CID | |
| PubChem SIDs |
* Chemical properties calculated with Open Babel - O'Boyle et al. Open Babel: An open chemical toolbox. J Cheminform 3, 33 (2011) (link)
Download Structures
| 2D | .png .mol .mol2 .sdf .pdb .smi |
| 3D | .mol .mol2 .sdf .pdb |
Tautomers
| Tautomers SMILES |
COC(N=C(C(OC)O)CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)=O tautomer #0
COC(NC(C(OC)=O)CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)=O tautomer #1 COC(NC(C(OC)O)=CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)=O tautomer #2 COC(NC(=C(OC)O)CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)=O tautomer #3 COC(=NC(C(OC)=O)CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)O tautomer #4 COC(=NC(=C(OC)O)CCc1n2c(n(c3c(c2=O)ncn3C4C(O)C(O)C(CO)O4)C)nc1C)O tautomer #5 |
| Tautomer image | Show Image |
Predicted CYP Metabolic Sites
| CYP3A4 | CYP2D6 | CYP2C9 |
|---|---|---|
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* CYP Metabolic sites predicted with SMARTCyp. SMARTCyp is a method for prediction of which sites in a molecule that are most liable to metabolism by Cytochrome P450. It has been shown to be applicable to metabolism by the isoforms 1A2, 2A6, 2B6, 2C8, 2C19, 2E1, and 3A4 (CYP3A4), and specific models for the isoform 2C9 (CYP2C9) and isoform 2D6 (CYP2D6). CYP3A4, CYP2D6, and CYP2C9 are the three of the most important enzymes in drug metabolism since they are involved in the metabolism of more than half of the drugs used today. The three top-ranked atoms are highlighted. See: SmartCYP and SmartCYP - background; Patrik Rydberg, David E. Gloriam, Lars Olsen, The SMARTCyp cytochrome P450 metabolism prediction server, Bioinformatics, Volume 26, Issue 23, 1 December 2010, Pages 2988–2989 (link)
LC-MS Information
| Monoisotopic mass | 508.1918 |
| Average mass | 508.482 |
| [M+H]+ | 509.1996 |
| Product ions | 377 |
| Normalized LC elution time * | 2,93 (Kellner 2014) |
| LC elution order/characteristics | after m6A (Kellner 2014) |
* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.
LC-MS Publications
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| Profiling of RNA modifications by multiplexed stable isotope labelling. | Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. | Chem Commun (Camb). | [details] | 24567952 | - |
Chemical groups contained
| Type | Subtype |
|---|---|
| aminoacyl group | glutamyl |
| other | acetylamide |
| other | methylester |
| ring modification | wyosine |
Reactions producing wybutosine
| Name |
|---|
| yW-58:yW |
Publications
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA. | Rajbhandary UL, Chang SH, Stuart A, Faulkner RD, Hoskinson RM, Khorana HG | Proc Natl Acad Sci U S A | [details] | 16591527 | - |
| A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid. | Thiebe R, Zachau HG | Eur J Biochem | [details] | 5698615 | - |
| A fluorescence assay for phenylalanine transfer RNA. | Yoshikami D, Katz G, Keller EB, Dudock BS | Biochim Biophys Acta | [details] | 5722700 | - |
Last modification of this entry: Sept. 22, 2023