Full name: | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase |
---|---|
Synonym: | YleA |
GI: | 83288203 |
Orf: | b0661 |
COG: | COG0621 |
UniProt: | P0AEI1 |
Alpha Fold Predicted Structure: | AF-P0AEI1-F1 |
Enzyme type: | methylthiotransferase |
Position of modification - modification: |
t:37 - ms2i6A |
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
Bacterial MiaB works on A36-i6A37-containing tRNA. It displays a N-terminal TRAM domain and a C-terminal catalytic radical SAM domain with two [4Fe-4S] clusters coordinated with 3 cysteines and an exchangeable S-AdoMet. It works with IcsS/IcsU as the donor of sulfur. Belongs to the same family of methylthiotransferases as B subtilis MTaB (YqeV), mammalian MtaB (Cdkal1) and protein methylthiotransferase RimO. A MiaB-CDKAL1-like enzyme is present in Archaea, while MiaA/Mod5 is totally absent. Its function is still elusive (2012). MnmE-GTpase negatively regulates the activity of MiaG in E. coli.
Reaction | Substrate | SubstrateType | Position | (Anti)Codon | Modified (Anti)Codon | Amino Acid Change | Transcript Name | Transcript Region | Cellular Localization | References |
---|---|---|---|---|---|---|---|---|---|---|
i6A:ms2i6A | RNA | tRNA | 37 | GCA | GCA | tRNACysGCA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | UAA | UAA | tRNALeuUAA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | GAA | GAA | tRNAPheGAA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | UGA | UGA | tRNASerUGA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | CGA | CGA | tRNASerCGA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | CCA | CCA | tRNATrpCCA | anticodon | Prokaryotic Cytosol | ||
i6A:ms2i6A | RNA | tRNA | 37 | GUA | GUA | tRNATyrGUA | anticodon | Prokaryotic Cytosol |
Alpha Fold Pdb Files | AF-P0AEI1-F1.pdb   |
Alpha Fold Pdbx/mmCIF Files | AF-P0AEI1-F1.cif   |
DSSP Secondary Structures | P0AEI1.dssp   |
Title | Authors | Journal | Details | ||
---|---|---|---|---|---|
MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA. | Pierrel F, Douki T, Fontecave M, Atta M | J Biol Chem | [details] | 15339930 | - |
Identification of the miaB gene, involved in methylthiolation of isopentenylated A37 derivatives in the tRNA of Salmonella typhimurium and Escherichia coli. | Esberg B, Leung HC, Tsui HC, Bjork GR, Winkler ME | J Bacteriol | [details] | 10572129 | - |
Formation of thiolated nucleosides present in tRNA from Salmonella enterica serovar Typhimurium occurs in two principally distinct pathways. | Leipuviene R, Qian Q, Bjork GR | J Bacteriol | [details] | 14729702 | - |
Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein. | Pierrel F, Bjork GR, Fontecave M, Atta M | J Biol Chem | [details] | 11882645 | - |
Evidence that the folate-dependent proteins YgfZ and MnmEG have opposing effects on growth and on activity of the iron-sulfur enzyme MiaB. | Waller JC, Ellens KW, Hasnain G, Alvarez S, Rocca JR, Hanson AD | J Bacteriol | [details] | 22081392 | - |
TRAM, a predicted RNA-binding domain, common to tRNA uracil methylation and adenine thiolation enzymes. | Anantharaman V, Koonin EV, Aravind L | FEMS Microbiol Lett | [details] | 11313137 | - |
_PubMed_ |