Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-specific adenosine deaminase subunit TAD2
Synonym: ADAT2
GI: 1352970
Orf: YJL035C
COG: COG0590
UniProt: P47058
Structures: | 7BV5 |
Alpha Fold Predicted Structure: AF-P47058-F1
Complex: Tad2/3
Enzyme type: deaminase
Position of modification - modification: t:34 - I


PDB Structures:


7BV5

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

The adenosine-to-inosine (A-to-I) editing in anticodons of tRNAs is critical for wobble base-pairing during translation. This modification is produced via deamination on A34 and catalyzed by the adenosine deaminase acting on tRNA (ADAT) enzyme. Eukaryotic ADATs are heterodimers composed of the catalytic subunit ADAT2 and the structural subunit ADAT3, but their molecular assemblies and catalytic mechanisms are largely unclear.

Download RCSB-PDB Structures:

Pdb Files   7BV5.pdb   7DIY.pdb  
Pdbx/mmCIF Files   7BV5.cif   7DIY.cif  


Protein sequence:

MQHIKHMRTAVRLARYALDHDETPVACIFVHTPTGQVMAYGMNDTNKSLTGVAHAEFMGIDQIKAMLGSRGVVDVFKDITLYVTVEPCIMCASALKQLDIGKVVFGCGNERFGGNGTVLSVNHDTCTLVPKNNSAAGYESIPGILRKEAIMLLRYFYVRQNERAPKPRSKSDRVLDKNTFPPMEWSKYLNEEAFIETFGDDYRTCFANKVDLSSNSVDWDLIDSHQDNIIQELEEQCKMFKFNVHKKSKV

Comments:

Tad2 is a part of the heterodimeric deaminase Tad2/3 (ADAT2/3) which is responsible for A:I reaction in tRNA position 34. Zinc enzyme.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:I RNA tRNA 34 IGC IGC tRNAAlaIGC anticodon-loop Cytoplasm
A:I RNA tRNA 34 ICG ICG tRNAArgICG anticodon-loop Cytoplasm
A:I RNA tRNA 34 IAU IAU tRNAIleIAU anticodon-loop Cytoplasm
A:I RNA tRNA 34 IGA IGA tRNASerIGA anticodon-loop Cytoplasm
A:I RNA tRNA 34 IGU IGU tRNAThrIGU anticodon-loop Cytoplasm
A:I RNA tRNA 34 IAC IAC tRNAValIAC anticodon-loop Cytoplasm
A:I RNA tRNA 34 IGG IGG tRNAProIGG anticodon-loop Cytoplasm 22024020   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M Q H I K H M R T A V R L A R Y A L D H D E T P V A C I F V H T P T G Q V M A Y G M N D T N K S L T G V A H A E F M G I D Q I K A M L G S R G V V D V F K D I T L Y V T V E P C I M C A S A L K Q L D I G K V V F G C G N E R F G G N G T V L S V N H D T C T L V P K N N S A A G Y E S I P G I L R K E A I M L L R Y F Y V R Q N E R A P K P R S K S D R V L D K N T F P P M E W S K Y L N E E A F I E T F G D D Y R T C F A N K V D L S S N S V D W D L I D S H Q D N I I Q E L E E Q C K M F K F N V H K K S K V

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P47058-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P47058-F1.cif  
DSSP Secondary Structures   P47058.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
An adenosine deaminase that generates inosine at the wobble position of tRNAs. Gerber AP, Keller W Science [details] 10550050 -
A-to-I and C-to-U editing within transfer RNAs. Su AA, Randau L Biochemistry (Mosc) [details] 22022967 -
Determinants of tRNA editing and modification: Avoiding conundrums, affecting function. Paris Z, Fleming IM, Alfonzo JD Semin Cell Dev Biol [details] 22024020 -

Links:

_Wikipedia_-_RNA_editing_
_Wikipedia_-_Deamination_
_SGD_