| Full name: |
Ribosomal RNA small subunit methyltransferase I |
| Synonym: |
yraL |
| GI: |
1789535 |
| Orf: |
|
| COG: |
COG0313 |
| UniProt: |
P67087 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
s:1403(1403) - m4Cm
|
Comments:
RsmI catalyzes 2’-O-ribose methylation of cytidine C1402 (Cm) in helix 44 within the decoding center of 16S rRNA. AdoMet is the methyl group donor and the 30S subunit (not the naked 16 rRNA) is the substrate. This enzyme is conserved in almost all species of bacteria. Together with methyltransferase TrmH, it allows the formation of very conserved m4Cm. Notice, in yeast, human and Arabidopsis the ribose methylation of equivalent C in 18S rRNA is catalyzed by a snoRNA-guided methylation machinery.
Protein sequence:
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINARLFALHDHNE
QQKAETLLAKLQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFC
YEGFLPAKSKGRRDALKAIEAEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPV
GELLAWVKEDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKY
ALEQQG
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
C:Cm
|
rRNA (r) |
SSU/16S/prokaryotic cytosol |
1402 |
|
m4C:m4Cm
|
rRNA (r) |
SSU/16S/prokaryotic cytosol |
1402 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA. |
Kimura S, Suzuki T |
Nucleic Acids Res |
[details]
|
19965768
|
-
|
| Influence of phylogeny on posttranscriptional modification of rRNA in thermophilic prokaryotes: the complete modification map of 16S rRNA of Thermus thermophilus. |
Guymon R, Pomerantz SC, Crain PF, McCloskey JA |
Biochemistry |
[details]
|
16605256
|
-
|
| Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli. |
Zhao M, Wang L, Zhang H, Dong Y, Gong Y, Zhang L, Wang J... |
Acta Crystallogr F Struct Biol Commun |
[details]
|
25195904
|
-
|