Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-specific adenosine deaminase 1
Synonym: ADAT1, YGL243W
GI: 1723979
Orf: HRA400
COG: COG0590
UniProt: P53065
Alpha Fold Predicted Structure: AF-P53065-F1
Enzyme type: deaminase
Position of modification - modification: t:37 - m1I



Protein sequence:

MVSCQGTRPCIVNLLTMPSEDKLGEEISTRVINEYSKLKSACRPIIRPSGIREWTILAGVAAINRDGGANKIEILSIATGVKALPDSELQRSEGKILHDCHAEILALRGANTVLLNRIQNYNPSSGDKFIQHNDEIPARFNLKENWELALYISRLPCGDASMSFLNDNCKNDDFIKIEDSDEFQYVDRSVKTILRGRLNFNRRNVVRTKPGRYDSNITLSKSCSDKLLMKQRSSVLNCLNYELFEKPVFLKYIVIPNLEDETKHHLEQSFHTRLPNLDNEIKFLNCLKPFYDDKLDEEDVPGLMCSVKLFMDDFSTEEAILNGVRNGFYTKSSKPLRKHCQSQVSRFAQWELFKKIRPEYEGISYLEFKSRQKKRSQLIIAIKNILSPDGWIPTRTDDVK

Comments:

ADAT1 is a homodimeric enzyme and shares conserved sequence motifs with other well-characterized adenosine deaminases, including mammalian mRNA deaminases (ADARs). Also, like most members of the deaminase superfamily, a Zn2+ ion is coordinated by a conserved histidine and two cysteines. The fourth ligand is an active water molecule that acts as the nucleophile. Highly conserved glutamate then aids the reactions by shuttling a proton from water to the N1 position of inosine during the deamination reaction. These motifs constitute the canonical deaminase signature (C/H)xExnPCxxC found in most nucleotide deaminases. Tad1 (ADAT1) catalyzes the A-to-I deamination which is the first step in m1I synthesis in tRNA position 37 (Paris et al. 2011 ).





Alpha Fold Predicted Structure:




Downloading... [212730/375238]


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Protein sequence:

M V S C Q G T R P C I V N L L T M P S E D K L G E E I S T R V I N E Y S K L K S A C R P I I R P S G I R E W T I L A G V A A I N R D G G A N K I E I L S I A T G V K A L P D S E L Q R S E G K I L H D C H A E I L A L R G A N T V L L N R I Q N Y N P S S G D K F I Q H N D E I P A R F N L K E N W E L A L Y I S R L P C G D A S M S F L N D N C K N D D F I K I E D S D E F Q Y V D R S V K T I L R G R L N F N R R N V V R T K P G R Y D S N I T L S K S C S D K L L M K Q R S S V L N C L N Y E L F E K P V F L K Y I V I P N L E D E T K H H L E Q S F H T R L P N L D N E I K F L N C L K P F Y D D K L D E E D V P G L M C S V K L F M D D F S T E E A I L N G V R N G F Y T K S S K P L R K H C Q S Q V S R F A Q W E L F K K I R P E Y E G I S Y L E F K S R Q K K R S Q L I I A I K N I L S P D G W I P T R T D D V K
50100150200250300350400SequenceGHITBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P53065-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P53065-F1.cif  
DSSP Secondary Structures   P53065.dssp  





Publications:

Links:

_Wikipedia_-_RNA_editing_
_Wikipedia_-_Deamination_
_SGD_