Modomics - A Database of RNA Modifications

Full name:	tRNA-specific adenosine deaminase, chloroplastic
Synonym:	AtTadA
GI:	15221490
Orf:	F14K14.18, F24J5.5
COG:	COG0590
UniProt:	Q9S7I0
Structures:	\| \|
Complex:
Enzyme type:	deaminase
Position of modification - modification:	t:34 - I

Comments:

A-to-I tRNA editing enzyme from chloroplasts. This enzyme has an unusual structure in that it harbors a large N-terminal domain of more than 1000 amino acids long which is not required for catalytic activity. Only the C-terminal region displays sequence similarity to the tRNA adenosine deaminase TadA from Escherichia coli. Moreover, AtTadA is imported into the chloroplast in vivo.

Protein sequence:

MFNTYTNSLQWPIRSRNQQDYCSLLPERSESYKLSKAYTSSRCYCVSSRSSCCCCCSTPSSSSFVKPKVLINPGFVLYGVRQSTLIQWPSFQRRLLVGGG
RLMGCEVYSSCDGIRRKNRSFKLRCLEESDECCGGRSCSDDVEAMISFLSEELIDEERKWNLVSRVKEKKKVGNVRKVSVEGSNSYGNGRVSQRVKKPEG
FGRRKEIKEDVKLNERYDCEHCGRRKKSSELESESRRGSKLVTGEYIGKSYRGDEEREVRPRRRKSSSCSSYYSLASSGEFESDTEDQEEDVEIYRENVR
SSEKKVVDQSAKRLKSRKEASQMHSRKKRDESSTGVDSRYQKQIFEEGENSNQAVTLNQRRRKKFSQTENRVSESTGNYEEDMEIHEVHVNDAETSSQNQ
KLFNEREDYRVHSIRNDSGNENIESSQHQLKERLETRYSSEDRVSEMRRRTKYSSSQEEGINVLQNFPEVTNNQQPLVEERISKQAGTRRTTEHISESSE
IHDIDIRNTYVSQREDQIRNQEVHAGLVSGLQSERKQQDYHIEHNPLQTTQSDRTSVSVSHTSDAVRYTEIQRKSEKRLIGQGSTTAVQSDSKVEKNGAQ
KEDSRLDHANSKKDGQTTLGLQSYQSKLSEEASSSQSSLMASRTKLQLVDLVSEEMQGSETTLIPPSSQLVSRRSGQSYRTGGVSIQEISHGTSESGYTT
AFEHPRAGASVNSQSAGELMGFTSHEDAMGSAHRLEQASEKYVGEFVKKAKHGVINPETEEQRAESNQLKRRDSRRSSGGSGAKGPSDEMWVTDSAQGTP
HPGATEGNAAVGNAIFKRNGRSLWNVIADIARLRWGSRAGSPDSSAKPAGRSSPNESVSSATWFSGREHDGSSDDNTKGDKVLPQEAPSLHQVEVGQTSP
RSQSEYPGTTKLKQRSERHEGVVSSPSSTILEGGSVSNRMSSTSGNQIVGVDEEEGGNFEFRLPETALTEVPMKLPSRNLIRSPPIKESSESSLTEASSD
QNFTVGEGRRYPRMDAGQNPLLFPGRNLRSPAVMEPPVPRPRMVSGSSSLREQVEQQQPLSAKSQEETGSVSADSALIQRKLQRNKQVVRDSFEEWEEAY
KVEAERRTVDEIFMREALVEAKKAADTWEVPVGAVLVHDGKIIARGYNLVEELRDSTAHAEMICIREGSKALRSWRLADTTLYVTLEPCPMCAGAILQAR
VNTLVWGAPNKLLGADGSWIRLFPGGEGNGSEASEKPPPPVHPFHPKMTIRRGVLESECAQTMQQFFQLRRKKKDKNSDPPTPTDHHHHHLPKLLNKMHQ
VLPFFCL

Enzymatic activities:

Reaction	Substrate	Type	Position
A:I	tRNA (t)	Arg/ICG/prokaryotic cytosol	34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme.	Karcher D, Bock R	RNA	[details]	19460869	-
Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG) and is essential for efficient chloroplast translation.	Delannoy E, Le Ret M, Faivre-Nitschke E, Estavillo GM, Bergdoll M, Taylor NL, Pogson BJ, Small I, Imbault P, Gualberto JM	Plant Cell	[details]	19602623	-
A-to-I and C-to-U editing within transfer RNAs.	Su AA, Randau L	Biochemistry (Mosc)	[details]	22022967	-
Determinants of tRNA editing and modification: Avoiding conundrums, affecting function.	Paris Z, Fleming IM, Alfonzo JD	Semin Cell Dev Biol	[details]	22024020	-

Links:

_Wikipedia - RNA editing_
_Wikipedia - Deamination_
_TAIR_

_Wikipedia - RNA editing_

_Wikipedia - Deamination_

_TAIR_