Full name: tRNA-specific adenosine deaminase, chloroplastic
Synonym: AtTadA
GI: 15221490
Orf: F14K14.18, F24J5.5
COG: COG0590
UniProt: Q9S7I0
Structures: | |
Complex:
Enzyme type: deaminase
Position of modification - modification: t:34 - I

Comments:

A-to-I tRNA editing enzyme from chloroplasts. This enzyme has an unusual structure in that it harbors a large N-terminal domain of more than 1000 amino acids long which is not required for catalytic activity. Only the C-terminal region displays sequence similarity to the tRNA adenosine deaminase TadA from Escherichia coli. Moreover, AtTadA is imported into the chloroplast in vivo.

Protein sequence:

MFNTYTNSLQWPIRSRNQQDYCSLLPERSESYKLSKAYTSSRCYCVSSRSSCCCCCSTPSSSSFVKPKVL INPGFVLYGVRQSTLIQWPSFQRRLLVGGGRLMGCEVYSSCDGIRRKNRSFKLRCLEESDECCGGRSCSD DVEAMISFLSEELIDEERKWNLVSRVKEKKKVGNVRKVSVEGSNSYGNGRVSQRVKKPEGFGRRKEIKED VKLNERYDCEHCGRRKKSSELESESRRGSKLVTGEYIGKSYRGDEEREVRPRRRKSSSCSSYYSLASSGE FESDTEDQEEDVEIYRENVRSSEKKVVDQSAKRLKSRKEASQMHSRKKRDESSTGVDSRYQKQIFEEGEN SNQAVTLNQRRRKKFSQTENRVSESTGNYEEDMEIHEVHVNDAETSSQNQKLFNEREDYRVHSIRNDSGN ENIESSQHQLKERLETRYSSEDRVSEMRRRTKYSSSQEEGINVLQNFPEVTNNQQPLVEERISKQAGTRR TTEHISESSEIHDIDIRNTYVSQREDQIRNQEVHAGLVSGLQSERKQQDYHIEHNPLQTTQSDRTSVSVS HTSDAVRYTEIQRKSEKRLIGQGSTTAVQSDSKVEKNGAQKEDSRLDHANSKKDGQTTLGLQSYQSKLSE EASSSQSSLMASRTKLQLVDLVSEEMQGSETTLIPPSSQLVSRRSGQSYRTGGVSIQEISHGTSESGYTT AFEHPRAGASVNSQSAGELMGFTSHEDAMGSAHRLEQASEKYVGEFVKKAKHGVINPETEEQRAESNQLK RRDSRRSSGGSGAKGPSDEMWVTDSAQGTPHPGATEGNAAVGNAIFKRNGRSLWNVIADIARLRWGSRAG SPDSSAKPAGRSSPNESVSSATWFSGREHDGSSDDNTKGDKVLPQEAPSLHQVEVGQTSPRSQSEYPGTT KLKQRSERHEGVVSSPSSTILEGGSVSNRMSSTSGNQIVGVDEEEGGNFEFRLPETALTEVPMKLPSRNL IRSPPIKESSESSLTEASSDQNFTVGEGRRYPRMDAGQNPLLFPGRNLRSPAVMEPPVPRPRMVSGSSSL REQVEQQQPLSAKSQEETGSVSADSALIQRKLQRNKQVVRDSFEEWEEAYKVEAERRTVDEIFMREALVE AKKAADTWEVPVGAVLVHDGKIIARGYNLVEELRDSTAHAEMICIREGSKALRSWRLADTTLYVTLEPCP MCAGAILQARVNTLVWGAPNKLLGADGSWIRLFPGGEGNGSEASEKPPPPVHPFHPKMTIRRGVLESECA QTMQQFFQLRRKKKDKNSDPPTPTDHHHHHLPKLLNKMHQVLPFFCL

Enzymatic activities:

Reaction Substrate Type Position
A:I tRNA (t) Arg/ICG/prokaryotic cytosol 34

Publications:

Title Authors Journal Details PubMed Id DOI
Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme. Karcher D, Bock R RNA [details] 19460869 -
Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG) and is essential for efficient chloroplast translation. Delannoy E, Le Ret M, Faivre-Nitschke E, Estavillo GM, Bergdoll M, Taylor NL, Pogson BJ, Small I, Imbault P, Gualberto JM Plant Cell [details] 19602623 -
A-to-I and C-to-U editing within transfer RNAs. Su AA, Randau L Biochemistry (Mosc) [details] 22022967 -
Determinants of tRNA editing and modification: Avoiding conundrums, affecting function. Paris Z, Fleming IM, Alfonzo JD Semin Cell Dev Biol [details] 22024020 -

Links:

_Wikipedia - RNA editing_
_Wikipedia - Deamination_
_TAIR_
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