Modomics - A Database of RNA Modifications

Full name:	Methylenetetrahydrofolate-tRNA-(uracil-54-)-methyltransferase TrmFO
Synonym:
GI:	55981411
Orf:
COG:	COG1206
UniProt:	Q5SID2
Structures:	\| 3G5Q \| 3G5R \| 3G5S \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:54 - m5s2U

Comments:

Homologue of B. subtilis TrmFO responsible for m5U formation in tRNA position 54. In T. thermophilus m5U in position 54 is further processed to m5s2U. Crystal structures of Thermus thermophilus TrmFO in its free form, tetrahydrofolate (THF)-bound form, and glutathione-bound form at 2.1-, 1.6-, and 1.05-A resolutions were solved. The activity was tested in vitro on tRNAIle transcript.

Protein sequence:

MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGETNAKGLLQAEMRRAGSLVMEAADLARVPAGGALAVDREEF
SGYITERLTGHPLLEVVREEVREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAASPIVLYESIDLTKCFRAGRYGQSADYLNCPMTEEEYRRFH
QALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFGPMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAE
IVRYGVMHRNTYLNAPRLLGETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARKALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLV
PPVEGRMGKKEKRQAMYRRGLEAFSAWLSGLNPPLPRPEAALV

Enzymatic activities:

Reaction	Substrate	Type	Position
U:m5U	tRNA (t)	Ile/GAU/prokaryotic cytosol	54

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase.	Nishimasu H, Ishitani R, Yamashita K, Iwashita C, Hirata A, Hori H, Nureki O	Proc Natl Acad Sci U S A	[details]	19416846	-
The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO).	Yamagami R, Yamashita K, Nishimasu H, Tomikawa C, Ochi A, Iwashita C, Hirata A, Ishitani R, Nureki O, Hori H...	J Biol Chem	[details]	23095745	-