Modomics - A Database of RNA Modifications

Full name:	tRNA (uracil(54)-C(5))-methyltransferase
Synonym:	PYRAB10780
GI:	14521283
Orf:	PAB0719
COG:	COG2265
UniProt:	Q9UZR7
Structures:	\| 2VS1 \| 2JJQ \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:54 - m5U

Comments:

The activity was tested in vitro on yeast tRNAAsp transcript

Protein sequence:

MRGVIRKLNDDGFGVLKGILVPFSAPGDEIIVERVERVKKRRVASQWKLVRSSPLRVGPRCKAFGKCGGCTLQHLNYDYQLEFKRKKLKRILGFEVEVVP
SPKIFGHRNRIDLAITKDGIGFRERGKWWKIVDIDECPVFGKTSREAIERLKEFIEEEKISVWNIKKDEGFLRYMVLREGKFTEEVMVNFVTKEGNLPDP
TNYFDFDSIYWSVNRSKSDVSYGDIERFWGKEFIRERLDDVDYLIHPNSFFQTNSYQAVNLVRKVSELVEGEKILDMYSGVGTFGIYLAKRGFNVKGFDS
NEFAIEMARRNVEINNVDAEFEVASDREVSVKGFDTVIVDPPRAGLHPRLVKRLNREKPGVIVYVSCNPETFARDVKMLDYRIDEIVALDMFPHTPHVEL
VAKLV

Enzymatic activities:

Reaction	Substrate	Type	Position
U:m5U	tRNA (t)	Asp/GUC/prokaryotic cytosol	54

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)-methyltransferase provides insights into its tRNA specificity.	Walbott H, Leulliot N, Grosjean H, Golinelli-Pimpaneau B	Nucleic Acids Res	[details]	18653523	-
Acquisition of a bacterial RumA-type tRNA(uracil-54, C5)-methyltransferase by Archaea through an ancient horizontal gene transfer.	Urbonavicius J, Auxilien S, Walbott H, Trachana K, Golinelli-Pimpaneau B, Brochier-Armanet C, Grosjean H	Mol Microbiol	[details]	18069966	-

Links:

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