ID Card:
Full name:
23S rRNA (uracil(747)-C(5))-methyltransferase
Synonym:
PYRAB11450
GI:
50401700
Orf:
PAB0760
COG:
COG2265
UniProt:
Q9UZK1
Alpha Fold Predicted Structure:
AF-Q9UZK1-F1
Enzyme type:
methyltransferase
Position of modification - modification:
l :887(747) - m5U
Protein sequence:
MRGIIKGVSNDGLGVLGEVLVPFAYPGDVVEVISTRERFGRTIARDFKLVKSSPIRVPGKCRYFGRCGGCLWQGLKYREQLKLKEEIFKRVTGVEAEIKGSPRIWFFRNISNFIVTVNGIGFKEFGMPRTVVSVDECPVFSERTKLYIRAMKRFLRETGLNPWNWKNGDVHYLQVREGKFTGEVMINVIAHIPPSGREELTEAFGFADSVYWSLKRDKRDDPKGIPTLIKGNEFIRESIEGLVYLIHPSTFFQTNSYALPILLKAVESFAEGSKVLDLYSGVGTFSLYLAKKGFEVTGVEVNEESVRVAKKSAEVNSLDVSFIPGRAEDAKLKGYETLIVDPPRKGLKDFSKRIAKEGPENLIYVSCNPSKFVLDYRNYLSKAYKIEDAVLIDMFPHTPHVEAVVKLRRR
Comments:
The gene coding for this enzyme appears in Thermococcales (Archaea) by lateral gene transfer of a bacterial gene coding for a RlmD (RumA-like). Once in Archaea, it duplicates into two paralogs (PAB0719 (TrmU54) and PAB0760). PAB0760 behaves like a bacterial RlmC (E. coli RumB) catalyzing in vitro the site-specific formation of m5U at position 747 (E. coli numbering) in helix 35 of domain II of P. abyssi 23S rRNA. A transcript of rRNA fragment encompassing helix 33-35 was used as substrate in an in vitro test system.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:m5U
RNA
rRNA
859
LSU-23S
Prokaryotic Cytosol
21051506
Alpha Fold Predicted Structure:
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Protein sequence:
M
R
G
I
I
K
G
V
S
N
D
G
L
G
V
L
G
E
V
L
V
P
F
A
Y
P
G
D
V
V
E
V
I
S
T
R
E
R
F
G
R
T
I
A
R
D
F
K
L
V
K
S
S
P
I
R
V
P
G
K
C
R
Y
F
G
R
C
G
G
C
L
W
Q
G
L
K
Y
R
E
Q
L
K
L
K
E
E
I
F
K
R
V
T
G
V
E
A
E
I
K
G
S
P
R
I
W
F
F
R
N
I
S
N
F
I
V
T
V
N
G
I
G
F
K
E
F
G
M
P
R
T
V
V
S
V
D
E
C
P
V
F
S
E
R
T
K
L
Y
I
R
A
M
K
R
F
L
R
E
T
G
L
N
P
W
N
W
K
N
G
D
V
H
Y
L
Q
V
R
E
G
K
F
T
G
E
V
M
I
N
V
I
A
H
I
P
P
S
G
R
E
E
L
T
E
A
F
G
F
A
D
S
V
Y
W
S
L
K
R
D
K
R
D
D
P
K
G
I
P
T
L
I
K
G
N
E
F
I
R
E
S
I
E
G
L
V
Y
L
I
H
P
S
T
F
F
Q
T
N
S
Y
A
L
P
I
L
L
K
A
V
E
S
F
A
E
G
S
K
V
L
D
L
Y
S
G
V
G
T
F
S
L
Y
L
A
K
K
G
F
E
V
T
G
V
E
V
N
E
E
S
V
R
V
A
K
K
S
A
E
V
N
S
L
D
V
S
F
I
P
G
R
A
E
D
A
K
L
K
G
Y
E
T
L
I
V
D
P
P
R
K
G
L
K
D
F
S
K
R
I
A
K
E
G
P
E
N
L
I
Y
V
S
C
N
P
S
K
F
V
L
D
Y
R
N
Y
L
S
K
A
Y
K
I
E
D
A
V
L
I
D
M
F
P
H
T
P
H
V
E
A
V
V
K
L
R
R
R
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases.
Auxilien S, Rasmussen A, Rose S, Brochier-Armanet C, Husson C, Fourmy D, Grosjean H, Douthwaite S
RNA
[details]
21051506
-
Links: