Modomics - A Database of RNA Modifications

Full name:	23S rRNA (uracil(747)-C(5))-methyltransferase
Synonym:	PYRAB11450
GI:	50401700
Orf:	PAB0760
COG:	COG2265
UniProt:	Q9UZK1
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	l:887(747) - m5U

Comments:

The gene coding for this enzyme appears in Thermococcales (Archaea) by lateral gene transfer of a bacterial gene coding for a RlmD (RumA-like). Once in Archaea, it duplicates into two paralogs (PAB0719 (TrmU54) and PAB0760). PAB0760 behaves like a bacterial RlmC (E. coli RumB) catalyzing in vitro the site-specific formation of m5U at position 747 (E. coli numbering) in helix 35 of domain II of P. abyssi 23S rRNA. A transcript of rRNA fragment encompassing helix 33-35 was used as substrate in an in vitro test system.

Protein sequence:

MRGIIKGVSNDGLGVLGEVLVPFAYPGDVVEVISTRERFGRTIARDFKLVKSSPIRVPGKCRYFGRCGGCLWQGLKYREQLKLKEEIFKRVTGVEAEIKG
SPRIWFFRNISNFIVTVNGIGFKEFGMPRTVVSVDECPVFSERTKLYIRAMKRFLRETGLNPWNWKNGDVHYLQVREGKFTGEVMINVIAHIPPSGREEL
TEAFGFADSVYWSLKRDKRDDPKGIPTLIKGNEFIRESIEGLVYLIHPSTFFQTNSYALPILLKAVESFAEGSKVLDLYSGVGTFSLYLAKKGFEVTGVE
VNEESVRVAKKSAEVNSLDVSFIPGRAEDAKLKGYETLIVDPPRKGLKDFSKRIAKEGPENLIYVSCNPSKFVLDYRNYLSKAYKIEDAVLIDMFPHTPH
VEAVVKLRRR

Enzymatic activities:

Reaction	Substrate	Type	Position
U:m5U	rRNA (r)	LSU/23S/prokaryotic cytosol	859

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases.	Auxilien S, Rasmussen A, Rose S, Brochier-Armanet C, Husson C, Fourmy D, Grosjean H, Douthwaite S	RNA	[details]	21051506	-

Links:

_PubMed_

_PubMed_