Modomics - A Database of RNA Modifications

Full name: 23S rRNA (uracil(747)-C(5))-methyltransferase
Synonym: PYRAB11450
GI: 50401700
Orf: PAB0760
COG: COG2265
UniProt: Q9UZK1
Structures: | |
Enzyme type: methyltransferase
Position of modification - modification: l:887(747) - m5U


The gene coding for this enzyme appears in Thermococcales (Archaea) by lateral gene transfer of a bacterial gene coding for a RlmD (RumA-like). Once in Archaea, it duplicates into two paralogs (PAB0719 (TrmU54) and PAB0760). PAB0760 behaves like a bacterial RlmC (E. coli RumB) catalyzing in vitro the site-specific formation of m5U at position 747 (E. coli numbering) in helix 35 of domain II of P. abyssi 23S rRNA. A transcript of rRNA fragment encompassing helix 33-35 was used as substrate in an in vitro test system.

Protein sequence:


Enzymatic activities:

Reaction Substrate Type Position
U:m5U rRNA (r) LSU/23S/prokaryotic cytosol 859


Title Authors Journal Details PubMed Id DOI
Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases. Auxilien S, Rasmussen A, Rose S, Brochier-Armanet C, Husson C, Fourmy D, Grosjean H, Douthwaite S RNA [details] 21051506 -



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