ID Card:
Full name:
23S rRNA (uracil-C(5))-methyltransferase RlmCD
Synonym:
YefA
GI:
50401309
Orf:
BSU06730
COG:
COG2265
UniProt:
O31503
Alpha Fold Predicted Structure:
AF-O31503-F1
Enzyme type:
methyltransferase
Position of modification - modification:
l :749(747) - m5U l :1968(1939) - m5U
Protein sequence:
MKMKPPVEKNEYYDVTFEDLTHEGAGVAKVQGFPIFVPNALPEEKAQIKVTRVKKGFAFGRLIELKEESPHRTDAPCPIYKQCGGCQLQHMTYEGQLLFKQKQVKDVLERIGKLDLSKVTVHPTLGMEDPWNYRNKAQVPVGEREGGLVAGFYQQRSHDIIDMSACLIQQSKNDEAVQAVKDICANYGVKAYNEERHKGWLRHIMVRYGVVTGEMMIVFITRTSDFPHKAKIIEDITAQFPHVKSIVQNINPNKTNVIFGNETNVIWGEEYIYDLIGDVKFAISARSFYQVNPEQTKVLYDKALEYAELQGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGNTNAEFAVGEAETVIPKWYEEGITADTLVVDPPRKGCDEALLRTIVEMKPKRVVYVSCNPGTLARDLRVLEDGGYVTREVQPVDMFPHTNHVECCVLIKLKE
Comments:
Methylation of U747 and U1939 in B. subtilis rRNA is catalysed by a single enzyme while in E. coli two separate enzymes (RlmC and RlmD) are responsible for these two modifications.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:m5U
RNA
rRNA
794
LSU-23S
Prokaryotic Cytosol
21824914
U:m5U
RNA
rRNA
1968
LSU-23S
Prokaryotic Cytosol
21824914
Alpha Fold Predicted Structure:
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Protein sequence:
M
K
M
K
P
P
V
E
K
N
E
Y
Y
D
V
T
F
E
D
L
T
H
E
G
A
G
V
A
K
V
Q
G
F
P
I
F
V
P
N
A
L
P
E
E
K
A
Q
I
K
V
T
R
V
K
K
G
F
A
F
G
R
L
I
E
L
K
E
E
S
P
H
R
T
D
A
P
C
P
I
Y
K
Q
C
G
G
C
Q
L
Q
H
M
T
Y
E
G
Q
L
L
F
K
Q
K
Q
V
K
D
V
L
E
R
I
G
K
L
D
L
S
K
V
T
V
H
P
T
L
G
M
E
D
P
W
N
Y
R
N
K
A
Q
V
P
V
G
E
R
E
G
G
L
V
A
G
F
Y
Q
Q
R
S
H
D
I
I
D
M
S
A
C
L
I
Q
Q
S
K
N
D
E
A
V
Q
A
V
K
D
I
C
A
N
Y
G
V
K
A
Y
N
E
E
R
H
K
G
W
L
R
H
I
M
V
R
Y
G
V
V
T
G
E
M
M
I
V
F
I
T
R
T
S
D
F
P
H
K
A
K
I
I
E
D
I
T
A
Q
F
P
H
V
K
S
I
V
Q
N
I
N
P
N
K
T
N
V
I
F
G
N
E
T
N
V
I
W
G
E
E
Y
I
Y
D
L
I
G
D
V
K
F
A
I
S
A
R
S
F
Y
Q
V
N
P
E
Q
T
K
V
L
Y
D
K
A
L
E
Y
A
E
L
Q
G
E
E
T
V
I
D
A
Y
C
G
I
G
T
I
S
L
F
L
A
K
Q
A
K
K
V
Y
G
V
E
I
V
P
E
A
I
E
D
A
K
R
N
A
E
L
N
G
N
T
N
A
E
F
A
V
G
E
A
E
T
V
I
P
K
W
Y
E
E
G
I
T
A
D
T
L
V
V
D
P
P
R
K
G
C
D
E
A
L
L
R
T
I
V
E
M
K
P
K
R
V
V
Y
V
S
C
N
P
G
T
L
A
R
D
L
R
V
L
E
D
G
G
Y
V
T
R
E
V
Q
P
V
D
M
F
P
H
T
N
H
V
E
C
C
V
L
I
K
L
K
E
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA.
Desmolaize B, Fabret C, Bregeon D, Rose S, Grosjean H, Douthwaite S
Nucleic Acids Res
[details]
21824914
-
Links: