Full name: |
23S rRNA (uracil-C(5))-methyltransferase RlmCD |
Synonym: |
YefA |
GI: |
50401309 |
Orf: |
BSU06730 |
COG: |
COG2265 |
UniProt: |
O31503 |
Structures: |
|
|
|
Complex: |
|
Enzyme type: |
methyltransferase |
Position of modification - modification: |
l:749(747) - m5U
l:1968(1939) - m5U
|
Comments:
Methylation of U747 and U1939 in B. subtilis rRNA is catalysed by a single enzyme while in E. coli two separate enzymes (RlmC and RlmD) are responsible for these two modifications.
Protein sequence:
MKMKPPVEKNEYYDVTFEDLTHEGAGVAKVQGFPIFVPNALPEEKAQIKVTRVKKGFAFGRLIELKEESP
HRTDAPCPIYKQCGGCQLQHMTYEGQLLFKQKQVKDVLERIGKLDLSKVTVHPTLGMEDPWNYRNKAQVP
VGEREGGLVAGFYQQRSHDIIDMSACLIQQSKNDEAVQAVKDICANYGVKAYNEERHKGWLRHIMVRYGV
VTGEMMIVFITRTSDFPHKAKIIEDITAQFPHVKSIVQNINPNKTNVIFGNETNVIWGEEYIYDLIGDVK
FAISARSFYQVNPEQTKVLYDKALEYAELQGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAK
RNAELNGNTNAEFAVGEAETVIPKWYEEGITADTLVVDPPRKGCDEALLRTIVEMKPKRVVYVSCNPGTL
ARDLRVLEDGGYVTREVQPVDMFPHTNHVECCVLIKLKE
Enzymatic activities:
Reaction
| Substrate
| Type
| Position
|
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
794 |
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
1968 |
Publications:
Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA. |
Desmolaize B, Fabret C, Bregeon D, Rose S, Grosjean H, Douthwaite S |
Nucleic Acids Res |
[details]
|
21824914
|
-
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Links: