Modomics - A Database of RNA Modifications

Full name:	23S rRNA (uracil-C(5))-methyltransferase RlmCD
Synonym:	YefA
GI:	50401309
Orf:	BSU06730
COG:	COG2265
UniProt:	O31503
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	l:749(747) - m5U l:1968(1939) - m5U

Comments:

Methylation of U747 and U1939 in B. subtilis rRNA is catalysed by a single enzyme while in E. coli two separate enzymes (RlmC and RlmD) are responsible for these two modifications.

Protein sequence:

MKMKPPVEKNEYYDVTFEDLTHEGAGVAKVQGFPIFVPNALPEEKAQIKVTRVKKGFAFGRLIELKEESPHRTDAPCPIYKQCGGCQLQHMTYEGQLLFK
QKQVKDVLERIGKLDLSKVTVHPTLGMEDPWNYRNKAQVPVGEREGGLVAGFYQQRSHDIIDMSACLIQQSKNDEAVQAVKDICANYGVKAYNEERHKGW
LRHIMVRYGVVTGEMMIVFITRTSDFPHKAKIIEDITAQFPHVKSIVQNINPNKTNVIFGNETNVIWGEEYIYDLIGDVKFAISARSFYQVNPEQTKVLY
DKALEYAELQGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGNTNAEFAVGEAETVIPKWYEEGITADTLVVDPPRKGCDEALLR
TIVEMKPKRVVYVSCNPGTLARDLRVLEDGGYVTREVQPVDMFPHTNHVECCVLIKLKE

Enzymatic activities:

Reaction	Substrate	Type	Position
U:m5U	rRNA (r)	LSU/23S/prokaryotic cytosol	794
U:m5U	rRNA (r)	LSU/23S/prokaryotic cytosol	1968

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA.	Desmolaize B, Fabret C, Bregeon D, Rose S, Grosjean H, Douthwaite S	Nucleic Acids Res	[details]	21824914	-

Links:

_PubMed_

_PubMed_