| Full name: |
23S rRNA (uracil-C(5))-methyltransferase RlmCD |
| Synonym: |
YefA |
| GI: |
50401309 |
| Orf: |
BSU06730 |
| COG: |
COG2265 |
| UniProt: |
O31503 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
l:749(747) - m5U
l:1968(1939) - m5U
|
Comments:
Methylation of U747 and U1939 in B. subtilis rRNA is catalysed by a single enzyme while in E. coli two separate enzymes (RlmC and RlmD) are responsible for these two modifications.
Protein sequence:
MKMKPPVEKNEYYDVTFEDLTHEGAGVAKVQGFPIFVPNALPEEKAQIKVTRVKKGFAFGRLIELKEESP
HRTDAPCPIYKQCGGCQLQHMTYEGQLLFKQKQVKDVLERIGKLDLSKVTVHPTLGMEDPWNYRNKAQVP
VGEREGGLVAGFYQQRSHDIIDMSACLIQQSKNDEAVQAVKDICANYGVKAYNEERHKGWLRHIMVRYGV
VTGEMMIVFITRTSDFPHKAKIIEDITAQFPHVKSIVQNINPNKTNVIFGNETNVIWGEEYIYDLIGDVK
FAISARSFYQVNPEQTKVLYDKALEYAELQGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAK
RNAELNGNTNAEFAVGEAETVIPKWYEEGITADTLVVDPPRKGCDEALLRTIVEMKPKRVVYVSCNPGTL
ARDLRVLEDGGYVTREVQPVDMFPHTNHVECCVLIKLKE
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
794 |
|
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
1968 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA. |
Desmolaize B, Fabret C, Bregeon D, Rose S, Grosjean H, Douthwaite S |
Nucleic Acids Res |
[details]
|
21824914
|
-
|
Links: