Modomics - A Database of RNA Modifications

Full name:	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
Synonym:	C14orf172, TRM61
GI:	123173772
Orf:
COG:	COG2519
UniProt:	Q96FX7
Structures:	\| 5CCB \| 5CD1 \| 5CCX \|
Complex:	Trm6/Trm61
Enzyme type:	methyltransferase
Position of modification - modification:	t:58 - m1A

Comments:

A part of heterodimeric Trm6/Trm61 MTase. The activity tested on yeast tRNAs in vitro (tRNAi Met) and on htRNA3Lys expressed in yeast cells in vivo. Paralog of mitochondrial Trm61B.

Protein sequence:

MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLE
LRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEG
GRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATKTPG

Enzymatic activities:

Reaction	Substrate	Type	Position
A:m1A	tRNA (t)	Lys/unknown/eukaryotic cytosol	58

Diseases connected to this enzyme:

Description	Reaction	Disease Name
TRM6/61 regulates the translation of a subset of mRNAs encoding proteins that play role in cancer.	A:m1A	Glioblastoma

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.	Ozanick S, Krecic A, Andersland J, Anderson JT	RNA	[details]	16043508	-
Crystal Structure of the Human tRNA mA58 Methyltransferase-tRNA Complex: Refolding of Substrate tRNA allows Access to the Methylation Target.	Finer-Moore J, Czudnochowski N, O'Connell JD 3rd, Wang AL, Stroud RM...	J Mol Biol	[details]	26470919	-