Modomics - A Database of RNA Modifications

ID Card:

Full name:	tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
GI:	222831587
COG:	COG2519
UniProt:	Q9BVS5
Structures:	\| 2B25 \|
Alpha Fold Predicted Structure:	AF-Q9BVS5-F1
Enzyme type:	methyltransferase
Position of modification - modification:	t:58 - m1A

PDB Structures:

2B25

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Download RCSB-PDB Structures:

Pdb Files	2B25.pdb
Pdbx/mmCIF Files	2B25.cif

Protein sequence:

MLMAWCRGPVLLCLRQGLGTNSFLHGLGQEPFEGARSLCCRSSPRDLRDGEREHEAAQRKAPGAESCPSLPLSISDIGTGCLSSLENLRLPTLREESSPRELEDSSGDQGRCGPTHQGSEDPSMLSQAQSATEVEERHVSPSCSTSRERPFQAGELILAETGEGETKFKKLFRLNNFGLLNSNWGAVPFGKIVGKFPGQILRSSFGKQYMLRRPALEDYVVLMKRGTAITFPKDINMILSMMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYKHWRDSWKLSHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHLKHGGVCAVYVVNITQVIELLDGIRTCELALSCEKISEVIVRDWLVCLAKQKNGILAQKVESKINTDVQLDSQEKIGVKGELFQEDDHEESHSDFPYGSFPYVARPVHWQPGHTAFLVKLRKVKPQLN

Comments:

Paralog of cytoplasmic Trm61A. Unlike its cytoplasmic paralog that consists of an alpha2beta2 heterotetramer formed by Trmt61A and Trmt6, Trmt61B forms a homo-oligomer (presumably a homotetramer).

Reaction	Substrate	SubstrateType	Position	(Anti)Codon	Modified (Anti)Codon	Amino Acid Change	Transcript Name	Transcript Region	Cellular Localization	References
A:m1A	RNA	tRNA	58	EAA	EAA		tRNA^Leu_EAA	TΨC-loop	Mitochondrion	23097428
A:m1A	RNA	tRNA	58	GCU	GCU		tRNA^Ser_GCU	TΨC-loop	Mitochondrion	23097428

Alpha Fold Predicted Structure:

Clear Selection and Reset Camera

Protein sequence:

M L M A W C R G P V L L C L R Q G L G T N S F L H G L G Q E P F E G A R S L C C R S S P R D L R D G E R E H E A A Q R K A P G A E S C P S L P L S I S D I G T G C L S S L E N L R L P T L R E E S S P R E L E D S S G D Q G R C G P T H Q G S E D P S M L S Q A Q S A T E V E E R H V S P S C S T S R E R P F Q A G E L I L A E T G E G E T K F K K L F R L N N F G L L N S N W G A V P F G K I V G K F P G Q I L R S S F G K Q Y M L R R P A L E D Y V V L M K R G T A I T F P K D I N M I L S M M D I N P G D T V L E A G S G S G G M S L F L S K A V G S Q G R V I S F E V R K D H H D L A K K N Y K H W R D S W K L S H V E E W P D N V D F I H K D I S G A T E D I K S L T F D A V A L D M L N P H V T L P V F Y P H L K H G G V C A V Y V V N I T Q V I E L L D G I R T C E L A L S C E K I S E V I V R D W L V C L A K Q K N G I L A Q K V E S K I N T D V Q L D S Q E K I G V K G E L F Q E D D H E E S H S D F P Y G S F P Y V A R P V H W Q P G H T A F L V K L R K V K P Q L N

Secondary Structure Alphabet

G: 3-turn helix (3₁₀helix)
H: α-helix
I: 𝝅-helix (5 - turn helix)
T: Hydrogen Bonded Turn
B: β-sheet
S: Bend
C: Coil (residues not present in any of the above conformations)
N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files	AF-Q9BVS5-F1.pdb
Alpha Fold Pdbx/mmCIF Files	AF-Q9BVS5-F1.cif
DSSP Secondary Structures	Q9BVS5.dssp

Diseases connected to this enzyme:

Description	Reaction	Disease Name
Missing m1A modification on mitochondrial tRNA at the 58 position is associated with the MERRF disease. m1A58 provides a positive charge on tRNA tertiary structure that affect the binding of elongation factor which delivers tRNA to ribosome.	A:m1A	Myoclonus epilepsy, ragged-red fibers (MERRF)

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs.	Chujo T, Suzuki T...	RNA	[details]	23097428	-

Links:

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