Modomics - A Database of RNA Modifications

Full name:	tRNA (uracil-5-)-methyltransferase
Synonym:	Nud1, Rnc1
GI:	110825750
Orf:	YKR056W
COG:	COG2265
UniProt:	P33753
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:54 - m5U

Comments:

AdoMet is the methyl group donor. Site-specific. Contains 1 TRAM domain.

Protein sequence:

MYEQFEFSFFFFENSDNKVKYKAHLISSIKRWSIITCMRCFWTVQKSIFKARFFACRNFVKKHNYKLISTMTGSTEMVPPTMKHTVDNKRLSSPLTDSGN
RRTKKPKLRKYKAKKVETTSPMGVLEFEVNDLLKSQNLSREQVLNDVTSILNDKSSTDGPIVLQYHREVKNVKVLEITSNGNGLALIDNPVETEKKQVVI
IPFGLPGDVVNIKVFKTHPYYVESDLLDVVEKSPMRRDDLIRDKYFGKSSGSQLEFLTYDDQLELKRKTIMNAYKFFAPRLVAEKLLPPFDTTVASPLQF
GYRTKITPHFDMPKRKQKELSVRPPLGFGQKGRPQWRKDTLDIGGHGSILDIDECVLATEVLNKGLTNERRKFEQEFKNYKKGATILLRENTTILDPSKP
TLEQLTEEASRDENGDISYVEVEDKKNNVRLAKTCVTNPRQIVTEYVDGYTFNFSAGEFFQNNNSILPIVTKYVRDNLQAPAKGDDNKTKFLVDAYCGSG
LFSICSSKGVDKVIGVEISADSVSFAEKNAKANGVENCRFIVGKAEKLFESIDTPSENTSVILDPPRKGCDELFLKQLAAYNPAKIIYISCNVHSQARDV
EYFLKETENGSAHQIESIRGFDFFPQTHHVESVCIMKRI

Enzymatic activities:

Reaction	Substrate	Type	Position
U:m5U	tRNA (t)	Ala/IGC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Arg/ACU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Arg/ICG/prokaryotic cytosol	54
U:m5U	tRNA (t)	Asn/GUU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Asp/GUC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Cys/GCA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Glu/CUC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Gly/GCC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Gly/{CC/prokaryotic cytosol	54
U:m5U	tRNA (t)	His/GUG/prokaryotic cytosol	54
U:m5U	tRNA (t)	Ile/IAU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Ile/UAU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Leu/UAG/prokaryotic cytosol	54
U:m5U	tRNA (t)	Leu/CAA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Leu/UAA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Lys/CUU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Lys/SUU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Met/CAU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Phe/GAA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Pro/UGG/prokaryotic cytosol	54
U:m5U	tRNA (t)	Ser/CGA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Ser/UGA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Ser/IGA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Thr/IGU/prokaryotic cytosol	54
U:m5U	tRNA (t)	Trp/CCA/prokaryotic cytosol	54
U:m5U	tRNA (t)	Val/CAC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Val/UAC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Val/IAC/prokaryotic cytosol	54
U:m5U	tRNA (t)	Arg/UCU/mitochondrion	54
U:m5U	tRNA (t)	Arg/ACG/mitochondrion	54
U:m5U	tRNA (t)	Gly/UCC/mitochondrion	54
U:m5U	tRNA (t)	His/GUG/mitochondrion	54
U:m5U	tRNA (t)	Ile/GAU/mitochondrion	54
U:m5U	tRNA (t)	Leu/UAA/mitochondrion	54
U:m5U	tRNA (t)	Lys/UUU/mitochondrion	54
U:m5U	tRNA (t)	Met/CAU/mitochondrion	54
U:m5U	tRNA (t)	Pro/UGG/mitochondrion	54
U:m5U	tRNA (t)	Ser/GCU/mitochondrion	54
U:m5U	tRNA (t)	Ser/UGA/mitochondrion	54
U:m5U	tRNA (t)	Thr/UAG/mitochondrion	54
U:m5U	tRNA (t)	Trp/UCA/mitochondrion	54
U:m5U	tRNA (t)	Tyr/GUA/mitochondrion	54
U:m5U	tRNA (t)		54

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae.	Nordlund ME, Johansson JO, von Pawel-Rammingen U, Bystrom AS	RNA	[details]	10864043	-