ID Card:
Full name:
23S rRNA (uracil(1939)-C(5))-methyltransferase RlmFO
Synonym:
Mcap0476
GI:
123535705
COG:
COG1206
UniProt:
Q2SS13
Alpha Fold Predicted Structure:
AF-Q2SS13-F1
Enzyme type:
methyltransferase
Position of modification - modification:
l :1947(1939) - m5U
Protein sequence:
MNKKVKIIGAGLAGCEAAYFLANNDIQVELYEVKTLIKNEVQKTNNFAELVCSNTFRSQSLLNAAGILKAEMRRLNSLVIKIADSCKIDGDDALAVDREDFSKKLTDVIKNHPNITIIEQNVSYIDDENDLTLIATGPLTTNELKEDIQRLIGKQKLFFMDASAPIITKDSIDFNKVYYSGRHKQGKYICCPLNEQEFNKFVDDLVNAEQVQLKEFEKSIFFKGCQPIEQLAKTSKKLLLKGPMSPNNLLDQNNQQPYAVVQLRQDDAKDSLYNMVGFQTNLKWPEQKRVFQTIPGLEKAKIVRYGVMHKNYYINSPKILNFKLQVIRKKNVFFAGQITGVEGYIESASSGIWAAINILAFINNKKIKPLPNTTILGALTNYITNSKIYSLKPMKCNLGILEQENKYQSNDKFYSYNNSKNSLENYIEQLNKILRTNI
Comments:
M. capricolum possesses two homologs of TrmFO - folate-dependent tRNA MTase. Mcap0476, renamed to RlmFO, is the only one which is active and was found to methylate U in position 1939 (E. coli numbering) in LSU rRNA. This enzyme represents the first folate-dependent flavoprotein seen to modify rRNA.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:m5U
RNA
rRNA
1947
LSU-23S
Prokaryotic Cytosol
24939895
Alpha Fold Predicted Structure:
Clear Selection and Reset Camera
Clear Selection
Reset Camera
Protein sequence:
M
N
K
K
V
K
I
I
G
A
G
L
A
G
C
E
A
A
Y
F
L
A
N
N
D
I
Q
V
E
L
Y
E
V
K
T
L
I
K
N
E
V
Q
K
T
N
N
F
A
E
L
V
C
S
N
T
F
R
S
Q
S
L
L
N
A
A
G
I
L
K
A
E
M
R
R
L
N
S
L
V
I
K
I
A
D
S
C
K
I
D
G
D
D
A
L
A
V
D
R
E
D
F
S
K
K
L
T
D
V
I
K
N
H
P
N
I
T
I
I
E
Q
N
V
S
Y
I
D
D
E
N
D
L
T
L
I
A
T
G
P
L
T
T
N
E
L
K
E
D
I
Q
R
L
I
G
K
Q
K
L
F
F
M
D
A
S
A
P
I
I
T
K
D
S
I
D
F
N
K
V
Y
Y
S
G
R
H
K
Q
G
K
Y
I
C
C
P
L
N
E
Q
E
F
N
K
F
V
D
D
L
V
N
A
E
Q
V
Q
L
K
E
F
E
K
S
I
F
F
K
G
C
Q
P
I
E
Q
L
A
K
T
S
K
K
L
L
L
K
G
P
M
S
P
N
N
L
L
D
Q
N
N
Q
Q
P
Y
A
V
V
Q
L
R
Q
D
D
A
K
D
S
L
Y
N
M
V
G
F
Q
T
N
L
K
W
P
E
Q
K
R
V
F
Q
T
I
P
G
L
E
K
A
K
I
V
R
Y
G
V
M
H
K
N
Y
Y
I
N
S
P
K
I
L
N
F
K
L
Q
V
I
R
K
K
N
V
F
F
A
G
Q
I
T
G
V
E
G
Y
I
E
S
A
S
S
G
I
W
A
A
I
N
I
L
A
F
I
N
N
K
K
I
K
P
L
P
N
T
T
I
L
G
A
L
T
N
Y
I
T
N
S
K
I
Y
S
L
K
P
M
K
C
N
L
G
I
L
E
Q
E
N
K
Y
Q
S
N
D
K
F
Y
S
Y
N
N
S
K
N
S
L
E
N
Y
I
E
Q
L
N
K
I
L
R
T
N
I
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA.
Lartigue C, Lebaudy A, Blanchard A, Yacoubi BE, Rose S, Grosjean H, Douthwaite S...
Nucleic Acids Res
[details]
24939895
-
Links: