| Full name: |
23S rRNA (uracil(1939)-C(5))-methyltransferase RlmFO |
| Synonym: |
Mcap0476 |
| GI: |
123535705 |
| Orf: |
|
| COG: |
COG1206 |
| UniProt: |
Q2SS13 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
l:1947(1939) - m5U
|
Comments:
M. capricolum possesses two homologs of TrmFO - folate-dependent tRNA MTase. Mcap0476, renamed to RlmFO, is the only one which is active and was found to methylate U in position 1939 (E. coli numbering) in LSU rRNA. This enzyme represents the first folate-dependent flavoprotein seen to modify rRNA.
Protein sequence:
MNKKVKIIGAGLAGCEAAYFLANNDIQVELYEVKTLIKNEVQKTNNFAELVCSNTFRSQSLLNAAGILKAEMRRLNSLVIKIADSCKIDGDDALAVDRED
FSKKLTDVIKNHPNITIIEQNVSYIDDENDLTLIATGPLTTNELKEDIQRLIGKQKLFFMDASAPIITKDSIDFNKVYYSGRHKQGKYICCPLNEQEFNK
FVDDLVNAEQVQLKEFEKSIFFKGCQPIEQLAKTSKKLLLKGPMSPNNLLDQNNQQPYAVVQLRQDDAKDSLYNMVGFQTNLKWPEQKRVFQTIPGLEKA
KIVRYGVMHKNYYINSPKILNFKLQVIRKKNVFFAGQITGVEGYIESASSGIWAAINILAFINNKKIKPLPNTTILGALTNYITNSKIYSLKPMKCNLGI
LEQENKYQSNDKFYSYNNSKNSLENYIEQLNKILRTNI
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
1947 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA. |
Lartigue C, Lebaudy A, Blanchard A, Yacoubi BE, Rose S, Grosjean H, Douthwaite S... |
Nucleic Acids Res |
[details]
|
24939895
|
-
|
Links:
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