Modomics - A Database of RNA Modifications

ID Card:

Full name: adenosine deaminase, tRNA specific 2
Synonym: dJ20N2.1, TAD2
GI: 74750199
UniProt: Q7Z6V5
Structures: | 3DH1 |
Alpha Fold Predicted Structure: AF-Q7Z6V5-F1
Enzyme type: deaminase


PDB Structures:


3DH1

Structure Description:

Title: Crystal structure of human tRNA-specific adenosine-34 deaminase subunit ADAT2
Classification: HYDROLASE
Technique: X-Ray Diffraction
Resolution: 2.8
R value free: 0.256
R value observed: 0.205
R value work: 0.202

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   3DH1.pdb  
Pdbx/mmCIF Files   3DH1.cif  


Protein sequence:

MEAKAAPKPAASGACSVSAEETEKWMEEAMHMAKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNATRHAEMVAIDQVLDWCRQSGKSPSEVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNIASADLPNTGRPFQCIPGYRAEEAVEMLKTFYKQENPNAPKSKVRKKECQKS

Comments:

Multiple mRNA codons can be decoded by a single tRNA via the post-transcriptional modification of tRNA wobble adenosine. In eukaryotes, adenosine-to-inosine modification is catalyzed by ADAR enzyme family (Savva et al. 2012 ). ADAT is a heterodimeric enzyme belonging to this family that acts on tRNAs. Evolutionary models exploring the sequence homology of ADAT and ADARs suggest these tRNA modifying enzymes be ancestral to ADARs enzymes. ADAT is composed of two subunits named ADAT2 and ADAT3. ADAT2 is the catalytic subunit of the ADAT complex. However, several ADAT3 residues are required for the ADAT complex to be functionally active (Ramos-Morales et al. 2021). Mutations in this enzyme cause different diseases, principally related to neurological disorders. ADAT complex modifies up to eight tRNA transcripts in their 34 anticodon adenosine. (Ramos-Morales et al. 2021)





Alpha Fold Predicted Structure:




Parsing response... [180291/180291]


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Protein sequence:

M E A K A A P K P A A S G A C S V S A E E T E K W M E E A M H M A K E A L E N T E V P V G C L M V Y N N E V V G K G R N E V N Q T K N A T R H A E M V A I D Q V L D W C R Q S G K S P S E V F E H T V L Y V T V E P C I M C A A A L R L M K I P L V V Y G C Q N E R F G G C G S V L N I A S A D L P N T G R P F Q C I P G Y R A E E A V E M L K T F Y K Q E N P N A P K S K V R K K E C Q K S
20406080100120140160180MEAKAAPKPAASGACSVSAEETEKWMEEAMHMAKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNATRHAEMVAIDQVLDWCRQSGKSPSEVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNIASADLPNTGRPFQCIPGYRAEEAVEMLKTFYKQENPNAPKSKVRKKECQKSSequenceHTBSN

Enter the variants

Position

Original

Variant

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q7Z6V5-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q7Z6V5-F1.cif  
DSSP Secondary Structures   Q7Z6V5.dssp  





Publications: