Modomics - A Database of RNA Modifications

ID Card:

Full name: adenosine deaminase, tRNA specific 2
Synonym: dJ20N2.1, TAD2
GI: 74750199
UniProt: Q7Z6V5
Structures: | 3DH1 |
Alpha Fold Predicted Structure: AF-Q7Z6V5-F1
Enzyme type: deaminase


PDB Structures:


3DH1

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   3DH1.pdb  
Pdbx/mmCIF Files   3DH1.cif  


Protein sequence:

MEAKAAPKPAASGACSVSAEETEKWMEEAMHMAKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNATRHAEMVAIDQVLDWCRQSGKSPSEVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNIASADLPNTGRPFQCIPGYRAEEAVEMLKTFYKQENPNAPKSKVRKKECQKS

Comments:

Multiple mRNA codons can be decoded by a single tRNA via the post-transcriptional modification of tRNA wobble adenosine. In eukaryotes, adenosine-to-inosine modification is catalyzed by ADAR enzyme family (Savva et al. 2012 ). ADAT is a heterodimeric enzyme belonging to this family that acts on tRNAs. Evolutionary models exploring the sequence homology of ADAT and ADARs suggest these tRNA modifying enzymes be ancestral to ADARs enzymes. ADAT is composed of two subunits named ADAT2 and ADAT3. ADAT2 is the catalytic subunit of the ADAT complex. However, several ADAT3 residues are required for the ADAT complex to be functionally active (Ramos-Morales et al. 2021). Mutations in this enzyme cause different diseases, principally related to neurological disorders. ADAT complex modifies up to eight tRNA transcripts in their 34 anticodon adenosine. (Ramos-Morales et al. 2021)




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:I tRNA (t)   34 AGT IGT tRNAThrAGT anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGC IGC tRNAAlaAGC anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGG IGG tRNAPro AGG anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGA IGA tRNASerAGA anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAG IAG tRNALeuAAG anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAT IAT tRNAIleAAT anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAC IAC tRNAValAAC anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 ACG ICG tRNAArgACG anticodon-loop Nucleoplasm 30590541   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M E A K A A P K P A A S G A C S V S A E E T E K W M E E A M H M A K E A L E N T E V P V G C L M V Y N N E V V G K G R N E V N Q T K N A T R H A E M V A I D Q V L D W C R Q S G K S P S E V F E H T V L Y V T V E P C I M C A A A L R L M K I P L V V Y G C Q N E R F G G C G S V L N I A S A D L P N T G R P F Q C I P G Y R A E E A V E M L K T F Y K Q E N P N A P K S K V R K K E C Q K S

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q7Z6V5-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q7Z6V5-F1.cif  
DSSP Secondary Structures   Q7Z6V5.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
RNA editing by adenosine deaminases generates RNA and protein diversity. Schaub M, Keller W... Biochimie [details] 12457566 -