Modomics - A Database of RNA Modifications

ID Card:

Full name: DExD-box helicase 21
Synonym: RH-II/GU, GURDB
GI: 76803555
UniProt: Q9NR30
Structures: | 2m3d | 6L5L | 6L5M | 6L5N | 6L5O |
Alpha Fold Predicted Structure: AF-Q9NR30-F1


PDB Structures:


2m3d

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   2m3d.pdb   6L5L.pdb   6L5M.pdb   6L5N.pdb   6L5O.pdb  
Pdbx/mmCIF Files   2m3d.cif   6L5L.cif   6L5M.cif   6L5N.cif   6L5O.cif  


Protein sequence:

MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Comments:

DDX21 is a DEAD-box RNA helicase that controls the transcriptional status of RNA polymerase I and II. In the nucleolus, DDX21 broadly and specifically localizes rDNA loci, binding to the transcribed region of rRNA but not to intergenic spacers. Besides, it binds various other types of RNAs such as snoRNAs, 7SK and mRNAs, even though to a lower extent (Calo et al. 2015). Indeed, it promotes rRNA transcription, modification and processing, being, moreover, required for rRNA-2’-O-methylation. Concerning this, it is probably involved in the recruitment of snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (Calo et al. 2015, Sloan et al. 2015). It participates in transcription regulation cleaving P-TEFb from 7SK snRNP so that this release is dependent on its helicase activity. Therefore, it promotes the transcription of its own target genes (Calo et al. 2015). It is also involved in the maintenance of DNA homeostasis, preventing damage to R-loops and trascription-associated genomic instability. Indeed, deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases (Song et al. 2017).





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M P G K L R S D A G L E S D T A M K K G E T L R K Q T E E K E K K E K P K S D K T E E I A E E E E T V F P K A K Q V K K K A E P S E V D M N S P K S K K A K K K E E P S Q N D I S P K T K S L R K K K E P I E K K V V S S K T K K V T K N E E P S E E E I D A P K P K K M K K E K E M N G E T R E K S P K L K N G F P H P E P D C N P S E A A S E E S N S E I E Q E I P V E Q K E G A F S N F P I S E E T I K L L K G R G V T F L F P I Q A K T F H H V Y S G K D L I A Q A R T G T G K T F S F A I P L I E K L H G E L Q D R K R G R A P Q V L V L A P T R E L A N Q V S K D F S D I T K K L S V A C F Y G G T P Y G G Q F E R M R N G I D I L V G T P G R I K D H I Q N G K L D L T K L K H V V L D E V D Q M L D M G F A D Q V E E I L S V A Y K K D S E D N P Q T L L F S A T C P H W V F N V A K K Y M K S T Y E Q V D L I G K K T Q K T A I T V E H L A I K C H W T Q R A A V I G D V I R V Y S G H Q G R T I I F C E T K K E A Q E L S Q N S A I K Q D A Q S L H G D I P Q K Q R E I T L K G F R N G S F G V L V A T N V A A R G L D I P E V D L V I Q S S P P K D V E S Y I H R S G R T G R A G R T G V C I C F Y Q H K E E Y Q L V Q V E Q K A G I K F K R I G V P S A T E I I K A S S K D A I R L L D S V P P T A I S H F K Q S A E K L I E E K G A V E A L A A A L A H I S G A T S V D Q R S L I N S N V G F V T M I L Q C S I E M P N I S Y A W K E L K E Q L G E E I D S K V K G M V F L K G K L G V C F D V P T A S V T E I Q E K W H D S R R W Q L S V A T E Q P E L E G P R E G Y G G F R G Q R E G S R G F R G Q R D G N R R F R G Q R E G S R G P R G Q R S G G G N K S N R S Q N K G Q K R S F S K A F G Q

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9NR30-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9NR30-F1.cif  
DSSP Secondary Structures   Q9NR30.dssp  





Diseases connected to this enzyme:

Description Reaction Disease Name
Loss of 2'-O-Me results in decreased leukemia self-renewal potential and reduced colony formation potential. N:Nm
Leukemia

Publications:

Title Authors Journal Details PubMed Id DOI