Modomics - A Database of RNA Modifications

ID Card:

Full name: Ribosomal RNA large subunit methyltransferase D
Synonym: RumA, YgcA
GI: 388478792
Orf: ygcA, b2776
COG: COG2265
UniProt: P55138
Structures: | 2BH2 | 1UWV |
Enzyme type: methyltransferase
Position of modification - modification: l:1939(1939) - m5U


PDB Structures:


2BH2

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

RumA catalyzes transfer of a methyl group from S-adenosylmethionine (SAM) specifically to uridine 1939 of 23S ribosomal RNA in Escherichia coli to yield 5-methyluridine. We determined the crystal structure of RumA at 1.95 A resolution. The protein is organized into three structural domains: The N-terminal domain contains sequence homology to the conserved TRAM motif and displays a five-stranded beta barrel architecture characteristic of an oligosaccharide/oligonucleotide binding fold. The central domain contains a [Fe(4)S(4)] cluster coordinated by four conserved cysteine residues. The C-terminal domain displays the typical SAM-dependent methyltransferase fold. The catalytic nucleophile Cys389 lies in a motif different from that in DNA 5-methylcytosine methyltransferases. The electrostatic potential surface reveals a predominately positively charged area that covers the concave surface of the first two domains and suggests an RNA binding mode. The iron-sulfur cluster may be involved in the correct folding of the protein or may have a role in RNA binding.

Download RCSB-PDB Structures:

Pdb Files   1UWV.pdb   2BH2.pdb  
Pdbx/mmCIF Files   1UWV.cif   2BH2.cif  


Protein sequence:

MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQRSKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVKQCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVARALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQNVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK

Comments:

RlmD (formerly RumA) methylates the conserved U1939 in a loose hairpin ‘70’ located between conserved hairpins 69 and 71 in domain IV the large subunit rRNA. Recombinant E.coli RlmD was shown to be active in vitro on a small 40 nucleotides long fragment of 23S rRNA encompassing U1939. It displays a N-terminal TRAM domain, a five-stranded beta barrel architecture characteristic of an oligosaccharide/ oligonucleotide binding fold and a central domain contains a [Fe(4)S(4)] cluster coordinated by four conserved cysteine residues. The C-terminal domain displays the typical SAM-dependent methyltransferase fold. AdoMet is the methyl group donor. The paralog enzyme in B. subtilis (RlmCD) is multisite specific, while in Archaea, no RlmD paralog has been identified. The presence of m5U1939 methylation in 23S rRNA is not essential but helps to improve ribosome performance.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:m5U RNA rRNA 1939 LSU-23S Prokaryotic Cytosol 20067766   



Publications:

Title Authors Journal Details PubMed Id DOI
Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli. Agarwalla S, Kealey JT, Santi DV, Stroud RM J Biol Chem [details] 11779873 -
A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function. Lee TT, Agarwalla S, Stroud RM Cell [details] 15766524 -
Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase. Lee TT, Agarwalla S, Stroud RM Structure [details] 15016356 -
Mutagenesis of the modified bases, m(5)U1939 and psi2504, in Escherichia coli 23S rRNA. Persaud C, Lu Y, Vila-Sanjurjo A, Campbell JL, Finley J, O'Connor M Biochem Biophys Res Commun [details] 20067766 -