ID Card:
Full name:
Ribosomal RNA large subunit methyltransferase C
Synonym:
YbgF, RumB
GI:
2501407
Orf:
ybgF, b0859
COG:
COG2265
UniProt:
P75817
Alpha Fold Predicted Structure:
AF-P75817-F1
Enzyme type:
methyltransferase
Position of modification - modification:
l :747(747) - m5U
Protein sequence:
MQCALYDAGRCRSCQWIMQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHEQLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAAELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ
Comments:
RlmC (formerly RumB) methylates the conserved U747 in the loop of helix 35 of domain II of the large subunit rRNA. Recombinant E.coli RlmC is inactive in vitro. However, its archaeal recombinant RlmC paralog (P. abyssi PAB0760) is active on transcript corresponding to a fragment of rRNA encompassing the hairpin 33-35. AdoMet is the methyl group donor.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:m5U
rRNA (r)
LSU/23S/prokaryotic cytosol
747
LSU-23S
Prokaryotic Cytosol
12907714
Alpha Fold Predicted Structure:
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Protein sequence:
M
Q
C
A
L
Y
D
A
G
R
C
R
S
C
Q
W
I
M
Q
P
I
P
E
Q
L
S
A
K
T
A
D
L
K
N
L
L
A
D
F
P
V
E
E
W
C
A
P
V
S
G
P
E
Q
G
F
R
N
K
A
K
M
V
V
S
G
S
V
E
K
P
L
L
G
M
L
H
R
D
G
T
P
E
D
L
C
D
C
P
L
Y
P
A
S
F
A
P
V
F
A
A
L
K
P
F
I
A
R
A
G
L
T
P
Y
N
V
A
R
K
R
G
E
L
K
Y
I
L
L
T
E
S
Q
S
D
G
G
M
M
L
R
F
V
L
R
S
D
T
K
L
A
Q
L
R
K
A
L
P
W
L
H
E
Q
L
P
Q
L
K
V
I
T
V
N
I
Q
P
V
H
M
A
I
M
E
G
E
T
E
I
Y
L
T
E
Q
Q
A
L
A
E
R
F
N
D
V
P
L
W
I
R
P
Q
S
F
F
Q
T
N
P
A
V
A
S
Q
L
Y
A
T
A
R
D
W
V
R
Q
L
P
V
K
H
M
W
D
L
F
C
G
V
G
G
F
G
L
H
C
A
T
P
D
M
Q
L
T
G
I
E
I
A
S
E
A
I
A
C
A
K
Q
S
A
A
E
L
G
L
T
R
L
Q
F
Q
A
L
D
S
T
Q
F
A
T
A
Q
G
D
V
P
E
L
V
L
V
N
P
P
R
R
G
I
G
K
P
L
C
D
Y
L
S
T
M
A
P
R
F
I
I
Y
S
S
C
N
A
Q
T
M
A
K
D
I
R
E
L
P
G
F
R
I
E
R
V
Q
L
F
D
M
F
P
H
T
A
H
Y
E
V
L
T
L
L
V
K
Q
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry.
Madsen CT, Mengel-Jorgensen J, Kirpekar F, Douthwaite S
Nucleic Acids Res
[details]
12907714
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