| Full name: |
Ribosomal RNA large subunit methyltransferase C |
| Synonym: |
YbgF, RumB |
| GI: |
2501407 |
| Orf: |
ybgF, b0859 |
| COG: |
COG2265 |
| UniProt: |
P75817 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
l:747(747) - m5U
|
Comments:
RlmC (formerly RumB) methylates the conserved U747 in the loop of helix 35 of domain II of the large subunit rRNA. Recombinant E.coli RlmC is inactive in vitro. However, its archaeal recombinant RlmC paralog (P. abyssi PAB0760) is active on transcript corresponding to a fragment of rRNA encompassing the hairpin 33-35. AdoMet is the methyl group donor.
Protein sequence:
MQCALYDAGRCRSCQWIMQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAA
LKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHEQLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFND
VPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAAELGLTRLQFQALDSTQFATAQGDV
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
U:m5U
|
rRNA (r) |
LSU/23S/prokaryotic cytosol |
747 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry. |
Madsen CT, Mengel-Jorgensen J, Kirpekar F, Douthwaite S |
Nucleic Acids Res |
[details]
|
12907714
|
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|
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