Modomics - A Database of RNA Modifications

Full name:	tRNA (cytidine(32)/uridine(32)-2′-O)-methyltransferase
Synonym:	YfhQ, JW2516
GI:	83287928
Orf:	b2532
COG:	COG0565
UniProt:	P0AE01
Structures:	\| 4CND \| 4CNE \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:32 - Um t:32 - Cm

Comments:

AdoMet is the methyl group donor.

Protein sequence:

MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKS
VAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFI
RENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNKGNKAE

Enzymatic activities:

Reaction	Substrate	Type	Position
C:Cm	tRNA (t)	Ser/UGA/prokaryotic cytosol	32
C:Cm	tRNA (t)	Gln/CUG/prokaryotic cytosol	32
U:Um	tRNA (t)	Ser/UGA/prokaryotic cytosol	32
U:Um	tRNA (t)	Gln/CUG/prokaryotic cytosol	32

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase.	Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM	BMC Mol Biol	[details]	16848900	-
Characterization of two homologous 2'-O-methyltransferases showing different specificities for their tRNA substrates.	Somme J, Van Laer B, Roovers M, Steyaert J, Versees W, Droogmans L...	RNA	[details]	24951554	-
tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily.	Liu RJ, Long T, Zhou M, Zhou XL, Wang ED	Nucleic Acids Res.	[details]	26202969	10.1093/nar/gkv745