Modomics - A Database of RNA Modifications

Full name:	tRNA-2′-O-methyltransferase TRM13
Synonym:
GI:	6324447
Orf:	YOL125w
COG:	COG2230
UniProt:	Q12383
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:4 - Cm t:4 - Am

Comments:

The enzyme and the modification position is conserved in eukaryotes.

Protein sequence:

MLQDNNGPAVKRAKPSERLQCEYFMEKKKRRCGMTRSSQNLYCSEHLNLMKKAANSQVHNKNGSEAEKER ERVPCPLDPNHTVWADQLKKHLKKCNKTKLSHLNDDKPYYEPGYNGENGLLSSSVKIDITAEHLVQSIEL LYKVFEGESMDELPLRQLNNKLMSLKRFPQLPSNTKHAVQQSSLIENLVDAGAFERPESLNFIEFGCGRA EFSRYVSLYLLTQLTSLPAEHSGSNSNEFVLIDRATNRMKFDKKIKDDFSEIKSNSPSKPISCPSIKRIK IDIRDLKMDPILKSTPGDDIQYVCISKHLCGVATDLTLRCIGNSSILHGDDNNGCNPKLKAICIAMCCRH VCDYGDYVNRSYVTSLVEKYRAHGSILTYETFFRVLTKLCSWGTCGRKPGTAITDIVNVVESFEGAEPYT ITIKERENIGLMARRVIDEGRLVYVKEKFTEFNAELIRYVESDVSLENVAMLVYKK

Enzymatic activities:

Reaction	Substrate	Type	Position
C:Cm	tRNA (t)	Gly/GCC/prokaryotic cytosol	4
A:Am	tRNA (t)	His/GUG/cytosolic	4
C:Cm	tRNA (t)	Pro/UGG/cytosolic	4

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.	Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM	RNA	[details]	17242307	-
Trm13p, the tRNA:Xm4 modification enzyme from Saccharomyces cerevisiae is a member of the Rossmann-fold MTase superfamily: prediction of structure and active site.	Tkaczuk KL...	J Mol Model	[details]	19697067	-

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